CCL20/MIP-3 alpha Products

360-MP), Recombinant Mouse CCL20/MIP-3a (Catalog # 760-M3) and Recombinant Rat CCL20/MIP-3a (Catalog # 540-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CCL20/MIP-3a at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">

360-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP-3a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB360). The ND₅₀ is typically 0.2-2 µg/mL." class="big_lightbox" target="_blank">

360-MP) was serially diluted and captured by Mouse Anti-Human CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB360) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox" target="_blank">

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

360-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP‑3a (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL20/MIP‑3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_lightbox" target="_blank">

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to myoid cells, spermatogonia and spermatids.. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">

360-MP) was serially diluted and captured by Mouse Anti-Human CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB360) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

760-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL20/MIP-3a (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB7601). The ND₅₀ is typically 3-15 µg/mL." class="big_lightbox" target="_blank">

760-M3) was serially diluted and captured by Rat Anti-Mouse CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB7601) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF760) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">