CCL20/MIP-3 alpha Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL20/MIP-3 alpha Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse CCL20/MIP-3 alpha (R&D Systems, Catalog # 760-M3)
  Ala27-Met96
  Accession # Q642U4
• Specificity: Detects mouse MIP-3 alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) MIP-3 alpha , rhMIP-3 beta , recombinant mouse (rm) MIP-3 beta , recombinant rat MIP-3 alpha , rmMIP-1 gamma , rmMIP-1 alpha , rmMIP-1 beta , and rmMIP-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL20
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Mouse CCL20/MIP-3 alpha Antibody (Catalog # MAB7601)
  ELISA Detection: Mouse CCL20/MIP-3 alpha Biotinylated Antibody (Catalog # BAF760)
  Standard: Recombinant Mouse CCL20/MIP-3 alpha (Catalog # 760-M3)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

MIP-3 alpha , also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20 - 28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71 and 63% amino acid sequence homology with rat and human MIP-3 alpha , respectively.

MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34⁺ cord blood precursors.

1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
2. Hromas, R. et al. (1997) Blood 89:3315.
3. Greaves, D. R. et al. (1997) J. Exp. Med. 186: 857.
4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL20