CCL19/MIP-3 beta Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL19/MIP-3 beta Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse CCL19/MIP-3 beta (R&D Systems, Catalog # 440-M3)
  Gly26-Val107
  Accession # Q548P0
• Specificity: Detects mouse CCL19/MIP-3 beta in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) TECK, rmTARC, recombinant human MIP-3 beta , rmMIP-1 alpha , rmMIP-1 beta , rm6Ckine, and rmRANTES is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ccl19
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Mouse CCL19/MIP-3 beta Antibody (Catalog # MAB880)
  ELISA Detection: Mouse CCL19/MIP-3 beta Biotinylated Antibody (Catalog # BAF880)
  Standard: Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

CCL19/MIP-3 beta , also known as ELC (EBI1-Ligand Chemokine), is a reported beta  chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse MIP-3 beta cDNA encodes a 108 amino acid residue precursor protein with a predicted 25 aa residue signal peptide that is cleaved to form the 83 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta . MIP-3 beta  has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B-cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7.

1. Kim, C.H. et al. (1998) J. Immunol. 160:2418.
2. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
3. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
4. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ccl19