![Sandwich ELISA: CCL16/HCC-4/LEC Antibody Pair [HRP] [NBP2-79474] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize2/CCL16-HCC-4-LEC-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79474-img0001.jpg "Sandwich ELISA: CCL16/HCC-4/LEC Antibody Pair [HRP] [NBP2-79474] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, S-ELISA |
| Conjugate | HRP |
| Standard Curve Range | 2.34-150 pg/ml |
| Sensitivity | 2.34 pg/ml |
| Description | Solid Phase sandwich ELISA for the quantitative determination of Human CCL16/HCC-4/LEC. |
| Specificity | This solid phase sandwich ELISA utilizes an antibody specific for Human CCL16/HCC-4/LEC. |
| Standard Curve Range | 2.34-150 pg/ml |
| Sensitivity | 2.34 pg/ml |
| Assay Type | Sandwich ELISA |
| Sample Volume | 100 ul |
| Gene | CCL16 |
| Application Notes | 1. Capture Antibody: Dilute to a working concentration of 2 ug/ml in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 150 pg/ml is recommended. |
| Storage | Storage is content dependent. |
| Components |
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![CCL16/HCC-4/LEC Antibody Pair [HRP]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "CCL16/HCC-4/LEC Antibody Pair [HRP]")
| HRP | NBP2-79474 |
Diseases for CCL16/HCC-4/LEC Antibody Pair (NBP2-79474)Discover more about diseases related to CCL16/HCC-4/LEC Antibody Pair (NBP2-79474).
| Pathways for CCL16/HCC-4/LEC Antibody Pair (NBP2-79474)View related products by pathway.
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| Gene Symbol | CCL16 |
![Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In C, a double label immunofluorescent image demonstrates a malignant serosal tumor between the liver and gall bladder that is lined by reactive mesothelium (solid arrow) which stains red for podoplanin as well as green for cytokeratins. The cells of the subjacent malignant serosal tumor stain weakly red for podoplanin. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol (2014)](http://images.novusbio.com/images2/Podoplanin Antibody (8.1.1)-Immunohistochemistry-Paraffin-NB600-1015-img0011.jpg)
![Western Blot: Podoplanin Antibody (8.1.1) [NB600-1015] - Analysis of Podoplanin in mouse kidney tissue extract.](http://images.novusbio.com/images2/Podoplanin-Antibody-(8.1.1)-Western-Blot-NB600-1015-img0001.jpg)
![Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) [NB600-1015] - Analysis of Podoplanin in mouse mammary gland after MDA-MB-231 orthotopic transplantation. Image courtesy of product review by Luana Schito.](http://images.novusbio.com/images2/Podoplanin-Antibody-(8.1.1)-Immunohistochemistry-Paraffin-NB600-1015-img0004.jpg)
![Western Blot: ATP7b Antibody [NB100-360] - ATP7b detected in 20 ug of mouse brain membrane fraction.](http://images.novusbio.com/images2/ATP7b Antibody-Western Blot-NB100-360-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NB100-360] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ATP7b [NB100-360] at a 1:100 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/ATP7b-Antibody-Immunocytochemistry-Immunofluorescence-NB100-360-img0005.jpg)
![Immunohistochemistry: ATP7b Antibody [NB100-360] - Analysis of ATP7b in mouse liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/images2/ATP7b-Antibody-Immunohistochemistry-NB100-360-img0004.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Western-Blot-NBP2-22203-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![ELISA: Human Ceruloplasmin ELISA Kit (Colorimetric) [NBP2-60616]](http://images.novusbio.com/images2/Human Ceruloplasmin ELISA Kit (Colorimetric)-ELISA-NBP2-60616-img0001.jpg)
![Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Simple-Western-NB500-106-img0008.jpg)
![Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. Immunofluorescence for PCNA (yellow) and DAPI staining (blue) on 30 um-cryosections of E32-P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 um. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fnana.2018.00029/full), licensed under a CC-BY licence.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Immunocytochemistry-Immunofluorescence-NB500-106-img0013.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Immunohistochemistry-Paraffin-NB500-106-img0007.jpg)
![Immunohistochemistry-Paraffin: CCR8 Antibody [NB100-709] - Section of mouse spleen.](http://images.novusbio.com/images2/CCR8-Antibody-Immunohistochemistry-Paraffin-NB100-709-img0002.jpg)