CCDC16 Antibody - Azide and BSA Free

Western Blot: CCDC16 Antibody [NBP2-97135] - Anti-CCDC16 rabbit polyclonal antibody at 1:500 dilution. Lane A: U251MG Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: NIH3T3 Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa

Immunohistochemistry-Paraffin: CCDC16 Antibody [NBP2-97135] - Immunochemical staining of human CCDC16 in human duodenum with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: CCDC16 Antibody [NBP2-97135] - CCDC16 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate Lane B: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-CCDC16 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CCDC16 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 49 kDa

Immunocytochemistry/Immunofluorescence: CCDC16 Antibody [NBP2-97135] - Immunofluorescence staining of CCDC16 in U251-MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97135 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CCDC16 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human CCDC16 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF830
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 5-10 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for CCDC16 Antibody - Azide and BSA Free

• CCDC16
• coiled-coil domain containing 16
• Coiled-coil domain-containing protein 16
• MGC20398
• OMCG1
• sel-13 suppressor of lin-12 homolog
• SEL13
• zinc finger protein 830

Background

CCDC16 (coiled-coil domain containing 16) is a U1-like C2H2-type zinc finger protein whose function has not been characterized. Coiled-coil proteins exhibit tertiary structures made up of a number of highly organized alpha helices wound around each other to mediate oligomerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF830