Cyclophilin-E Antibody (2F5) - Azide and BSA Free

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - PPIE monoclonal antibody (M02), clone 2F5. Analysis of PPIE expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: Cyclophilin-E Antibody (2F5) [H00010450-M02] - Analysis of monoclonal antibody to PPIE on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - PPIE monoclonal antibody (M02), clone 2F5. Analysis of PPIE expression in PC-12.

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - PPIE monoclonal antibody (M02), clone 2F5 Analysis of PPIE expression in Hela S3 NE.

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - PPIE monoclonal antibody (M02), clone 2F5. Analysis of PPIE expression in Raw 264.7.

Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - Analysis of PPIE expression in transfected 293T cell line by PPIE monoclonal antibody (M02), clone 2F5.Lane 1: PPIE transfected lysate(33.4 KDa).Lane 2: Non-transfected lysate.

ELISA: Cyclophilin-E Antibody (2F5) [H00010450-M02] - Detection limit for recombinant GST tagged PPIE is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 2F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cyclophilin-E Antibody (2F5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Cyclophilin-E Antibody (2F5) - Azide and BSA Free (H00010450-M02) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Cyclophilin-E Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PPIE (NP_006103, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAAAAIDNMNESELFGRTIRVNLAKPMRIKEGSSRPVWSDDD
• Specificity: PPIE - peptidylprolyl isomerase E (cyclophilin E)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPIE
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cyclophilin-E Antibody (2F5) - Azide and BSA Free

• Cyclophilin E
• cyclophilin-33
• CYP33
• CyP-33
• EC 5.2.1.8
• MGC111222
• MGC3736
• peptidyl-prolyl cis-trans isomerase E
• peptidylprolyl isomerase E (cyclophilin E)
• PPIase E
• Rotamase E

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activity. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclophilin-E Antibody (H00010450-M02)(1)

Publication using H00010450-M02

• Acosta J, Li Q, Freeburg NF et al. p53 restoration in small cell lung cancer identifies a latent cyclophilin-dependent necrosis mechanism Nature communications 2023-07-21 [PMID: 37479684]

Bioinformatics

• Gene Symbol: PPIE
• Entrez:
  • 10450(Human)
• OMIM:
  • 602435(Human)
• Uniprot:
  • NP_006103(Human)