Independent Antibodies: Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit ...read more
Immunohistochemistry-Paraffin: Cullin 3 Antibody [NB100-58788] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cul3 used at a dilution of 1:200 (1ug/ml). Detection: DAB
The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human cullin 3 using the numbering given in entry NP_003581.1 (GeneID 8452).
Predicted Species
Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CUL3
Purity
Immunogen affinity purified
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Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
88.93 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Cullin 3 is a member of the family of human Cullin genes (CUL-1, -2, -3, -4a, -4b and -5) homologous to the S. cerevisiae cdc53 gene. Cullin 3 forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by proteosomes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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