Cullin 3 Antibody

Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).

Immunohistochemistry-Paraffin: Cullin 3 Antibody [NB100-58788] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cul3 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cullin 3 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human cullin 3 using the numbering given in entry NP_003581.1 (GeneID 8452).
• Predicted Species: Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CUL3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Immunoprecipitation 2-5 ug/mg
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 88.93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Cullin 3 Overexpression Lysate

Reactivity Notes

X. laevis and X. tropicalis (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cullin 3 Antibody

• CUL3
• CUL-3
• cullin 3
• cullin-3
• KIAA0617
• NEDAUS
• PHA2E

Background

Cullin 3 is a member of the family of human Cullin genes (CUL-1, -2, -3, -4a, -4b and -5) homologous to the S. cerevisiae cdc53 gene. Cullin 3 forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by proteosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cullin 3 Antibody (NB100-58788)(5)

Publications using NB100-58788

• Lutz LM, Pace CR, Arnold MM. Rotavirus NSP1 Associates with Components of the Cullin RING Ligase Family of E3 Ubiquitin Ligases. J Virol 2016 Jul 1 [PMID: 27099313]
• Li C, Ao J, Fu J et al. Tumor-suppressor role for the SPOP ubiquitin ligase in signal-dependent proteolysis of the oncogenic co-activator SRC-3/AIB1. Oncogene 2011 Oct [PMID: 21577200]
• Hsia O Characterisation of the role of the NEDD8 E3 ligase DCNL5 in the apoptosis response Thesis Jan 1 2020 (IP)
• Chagraoui J, Girard S, Spinella JF, et al. UM171 Preserves Epigenetic Marks that Are Reduced in Ex Vivo Culture of Human HSCs via Potentiation of the CLR3-KBTBD4 Complex Cell stem cell Jan 7 2021 [PMID: 33417871] (WB)
• Brown KN Evidence of E3 Ubiquitin Ligase Activity in Rotavirus NSP1 Thesis Jul 13 1905 (IP)

Bioinformatics

• Gene Symbol: CUL3
• Entrez:
  • 8452(Human)
  • 26554(Mouse)
• Uniprot:
  • Q13618(Human)