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Cullin 3 Antibody

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Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).
Independent Antibodies: Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit ...read more
Immunohistochemistry: Cullin 3 Antibody [NB100-58788] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cul3 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, Mu, Rt, XpSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

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Cullin 3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human cullin 3 using the numbering given in entry NP_003581.1 (GeneID 8452).
Predicted Species
Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CUL3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 2-5 ug/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
88.93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Cullin 3 Lysate (NBL1-09608)
Publications
Read Publications using NB100-58788.

Reactivity Notes

X. laevis and X. tropicalis (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cullin 3 Antibody

  • CUL-3
  • cullin 3
  • cullin-3
  • KIAA0617

Background

Cullin 3 is a member of the family of human Cullin genes (CUL-1, -2, -3, -4a, -4b and -5) homologous to the S. cerevisiae cdc53 gene. Cullin 3 forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by proteosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cullin 3 Antibody (NB100-58788)(2)


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Control Lysate(s)

Cullin 3 Overexpression Lysate (Native)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CUL3
Entrez
Uniprot