Cathepsin E Antibody (186513)

• Reactivity: Mu
• Applications: WB
• Clone: 186513
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cathepsin E Antibody (186513) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin E
  Gln19-Pro397
  Accession # P70269
• Specificity: Detects mouse Cathepsin E in direct ELISAs and Western blots. It recognizes both the pro and active forms of recombinant mouse (rm) Cathepsin E. In Western blots, no cross-reactivity with rmCathepsin B, C, H, L, or recombinant human Cathepsin O, S, or V is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CTSE
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin E Antibody (186513)

• CATE
• Cathepsin E
• CTSE
• EC 3.4.23
• EC 3.4.23.34
• erythrocyte membrane aspartic proteinase
• slow-moving proteinase

Background

Cathepsin E is an intracellular aspartic protease of the pepsin family (1, 2). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsins D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (3). Both enzymes are efficient in cleaving the Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with the wild-type APP (4). Mouse Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1-18), a propeptide (residues 19-59), and a mature chain (residues 60-397) (1).

1. Tatnell, P.J. et al. (1997) FEBS Lett. 408:62.
2. Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.), p. 33,  Academic Press, San Diego.
3. Tsukuba, T. et al. (2000) Mol. Cells 10:601.
4. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin E Antibody (MAB1130)(1)

Publication using MAB1130

• Li XL, Ezelle HJ, Kang TJ, Zhang L, Shirey KA, Harro J, Hasday JD, Mohapatra SK, Crasta OR, Vogel SN, Cross AS, Hassel BA An essential role for the antiviral endoribonuclease, RNase-L, in antibacterial immunity. Proc. Natl. Acad. Sci. U.S.A., 2008-12-15;105(52):20816-21. 2008-12-15 [PMID: 19075243] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CTSE
• Uniprot:
  • P70269()