Cathepsin E Antibody (186513) [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB
• Clone: 186513
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

Cathepsin E Antibody (186513) [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin E
  Gln19-Pro397
  Accession # P70269
• Specificity: Detects mouse Cathepsin E in direct ELISAs and Western blots. It recognizes both the pro and active forms of recombinant mouse (rm) Cathepsin E. In Western blots, no cross-reactivity with rmCathepsin B, C, H, L, or recombinant human Cathepsin O,
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin E Antibody (186513) [Alexa Fluor® 532]

• CATE
• Cathepsin E
• CTSE
• EC 3.4.23
• EC 3.4.23.34
• erythrocyte membrane aspartic proteinase
• slow-moving proteinase

Background

Cathepsin E is an intracellular aspartic protease of the pepsin family (1, 2). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsins D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (3). Both enzymes are efficient in cleaving the Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with the wild-type APP (4). Mouse Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1-18), a propeptide (residues 19-59), and a mature chain (residues 60-397) (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.