Cathepsin A/Lysosomal Carboxypeptidase A Antibody (191430)

• Reactivity: Mu
• Applications: WB, IP
• Clone: 191430
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cathepsin A/Lysosomal Carboxypeptidase A Antibody (191430) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin A/Lysosomal Carboxypeptidase A
  Ala24-Tyr474 (predicted)
  Accession # P16675
• Specificity: Detects mouse Cathepsin A/Lysosomal Carboxypeptidase A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin A or recombinant mouse (rm) DPPIV/CD26 is observed. In Western blots, it recognizes the single chain (55 kDa) form of rmCathepsin A, which can be processed into heavy (32 kDa) and light (20 kDa) chains. It recognizes the heavy chain, but not the light chain.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CTSA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin A/Lysosomal Carboxypeptidase A Antibody (191430)

• beta-galactosidase 2
• beta-galactosidase protective protein
• Carboxypeptidase C
• Carboxypeptidase L
• Cathepsin A
• cathepsin ANGBE
• CTSA
• EC 3.4.16
• EC 3.4.16.5
• GLB2
• GSL
• Lysosomal Carboxypeptidase A
• lysosomal protective protein
• PPCA
• PPGBprotective protein for beta-galactosidase (galactosialidosis)
• Protective protein cathepsin A
• Protective protein for beta-galactosidase

Background

Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1, 2). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta -galactosidase ( beta -gal) and neuraminidase is essential for beta -gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta -gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal cathepsin A plays a role in regulation of functions of these molecules (3). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds.

1. Galjart, N.J. et al. (1990) J. Biol. Chem. 265:4678.
2. Pshezhetsky, A.V. (1998) in Handbook of Proteolytic Enzymes (ed. Barrett, Rawlings, Woessner) pp. 393, Academic Press, San Diego.
3. Hiraiwa, M. (1999) Cell. Mol. Life. Sci. 56:894.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTSA
• Uniprot:
  • P16675()