NEU-1/Sialidase-1 Antibody (688215)

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NEU‑1/Sialidase‑1 Monoclonal Antibody (Catalog # MAB6860) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEU‑1/Sialidase‑1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP
• Clone: 688215
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NEU-1/Sialidase-1 Antibody (688215) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NEU-1/Sialidase-1
  Lys46-Leu415
  Accession # Q99519
• Specificity: Detects human NEU-1/Sialidase-1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEU1
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NEU-1/Sialidase-1 Antibody (688215)

• Acetylneuraminyl hydrolase
• EC 3.2.1.18
• exo-alpha-sialidase
• FLJ93471
• G9 sialidase
• Lysosomal sialidase
• N-acetyl-alpha-neuraminidase 1
• NANH
• NEU
• NEU1
• NEU-1
• SIAL1
• sialidase 1 (lysosomal sialidase)
• Sialidase-1

Background

Neuraminidase, also called NEU-1 or lysosomal sialidase, is a 45 kDa glycoprotein in the N-acetyl-a neuraminidase family. It is a member of a complex within the secretory lysosomes of melanosomes and hematopoietic cells that removes terminal sialic acids from glycosylated proteins and lipids. Mature human NEU-1 (aa 46‑415) shares 86.5% aa identity with mouse and rat NEU-1. A 225 aa splice variant shares aa 206‑415, but has an alternate N-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NEU-1/Sialidase-1 Antibody (MAB6860)(1)

Publication using MAB6860

• Pawluczyk I, Ghaderi Najafabadi M, Patel S, Desai P, Vashi D, Saleem M, Topham P Sialic acid attenuates puromycin aminonucleoside-induced desialylation and oxidative stress in human podocytes. Exp Cell Res, 2013-11-04;320(2):258-68. 2013-11-04 [PMID: 24200502] (Western Blot, Human)

Review for NEU-1/Sialidase-1 Antibody (MAB6860) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/25/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24200502
• Species: Human

Bioinformatics

• Gene Symbol: NEU1
• Uniprot:
  • Q99519()