Cathepsin A/Lysosomal Carboxypeptidase A Antibody (191430) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB, IP
• Clone: 191430
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

Cathepsin A/Lysosomal Carboxypeptidase A Antibody (191430) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin A/Lysosomal Carboxypeptidase A
  Ala24-Tyr474 (predicted)
  Accession # P16675
• Specificity: Detects mouse Cathepsin A/Lysosomal Carboxypeptidase A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin A or recombinant mouse (rm) DPPIV/CD26 is observed. In
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin A/Lysosomal Carboxypeptidase A Antibody (191430) [Alexa Fluor® 488]

• beta-galactosidase 2
• beta-galactosidase protective protein
• Carboxypeptidase C
• Carboxypeptidase L
• Cathepsin A
• cathepsin ANGBE
• CTSA
• EC 3.4.16
• EC 3.4.16.5
• GLB2
• GSL
• Lysosomal Carboxypeptidase A
• lysosomal protective protein
• PPCA
• PPGBprotective protein for beta-galactosidase (galactosialidosis)
• Protective protein cathepsin A
• Protective protein for beta-galactosidase

Background

Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1, 2). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta -galactosidase ( beta -gal) and neuraminidase is essential for beta -gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta -gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal cathepsin A plays a role in regulation of functions of these molecules (3). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.