CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Cathepsin A/Lysosomal Carboxypeptidase A was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Cathepsin A/Lysosomal Carboxypeptidase A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1049) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1049-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89526-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98264-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11231-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1049-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1123-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98190-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21355-25ul | |||
![Immunocytochemistry/Immunofluorescence: Cathepsin A/Lysosomal Carboxypeptidase A Antibody (104) [NBP2-89526] - Staining of Human CTSA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89526 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89526_rabbit-monoclonal-cathepsin-a-lysosomal-carboxypeptidase-a-antibody-104-235202511444473.png "Immunocytochemistry/Immunofluorescence: Cathepsin A/Lysosomal Carboxypeptidase A Antibody (104) [NBP2-89526] - Staining of Human CTSA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89526 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Immunocytochemistry/Immunofluorescence: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cathepsin-A-Lysosomal-Carboxypeptidase-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89728-img0018.jpg "Immunocytochemistry/Immunofluorescence: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP1-89728] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.")
![Western Blot: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98264] - Lane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:28 kDa](http://images.novusbio.com/fullsize/Cathepsin-A-Lysosomal-Carboxypeptidase-A-Antibody-Western-Blot-NBP2-98264-img0002.jpg "Western Blot: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98264] - Lane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:28 kDa")
![Immunoprecipitation: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98264] - Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse CTSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CTSA rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 30 kDa](http://images.novusbio.com/fullsize/Cathepsin-A-Lysosomal-Carboxypeptidase-A-Antibody-Immunoprecipitation-NBP2-98264-img0001.jpg)
![ELISA: Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric) [NBP3-00371] - Samples were spiked with high concentrations of Human Cathepsin A/Lysosomal Carboxypeptidase A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00371_human-cathepsin-a-lysosomal-carboxypeptidase-a-elisa-kit-13220241692271.jpg "ELISA: Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric) [NBP3-00371] - Samples were spiked with high concentrations of Human Cathepsin A/Lysosomal Carboxypeptidase A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric) [NBP3-00371] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00371_human-cathepsin-a-lysosomal-carboxypeptidase-a-elisa-kit-432024915878.jpg)
![Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98190] - Staining of human Cathepsin A/Lysosomal Carboxypeptidase A in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Cathepsin-A-Lysosomal-Carboxypeptidase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-98190-img0002.jpg "Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98190] - Staining of human Cathepsin A/Lysosomal Carboxypeptidase A in human kidney with rabbit polyclonal antibody at 1:100 dilution.")
![Immunocytochemistry/Immunofluorescence: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98190] - Immunofluorescence staining of Cathepsin A/Lysosomal Carboxypeptidase A in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98190 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98190_rabbit-polyclonal-cathepsin-a-lysosomal-carboxypeptidase-a-antibody-235202514492385.png)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21355_rabbit-cathepsin-a-lysosomal-carboxypeptidase-a-pab-4520231550236.jpg "Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil")
![Western Blot: Cathepsin A Overexpression Lysate (Adult Normal) [NBL1-09586] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cathepsin A.](http://images.novusbio.com/fullsize/Cathepsin-A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09586-img0002.jpg "Western Blot: Cathepsin A Overexpression Lysate (Adult Normal) [NBL1-09586] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cathepsin A.")
