Caspase-9 Antibody

Images

 
Western Blot: Caspase-9 Antibody [NBP1-76961] - HeLa whole cell lysate with Caspase-9 antibody at 1:1000 dilution
Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Staining of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 ug/ml.
Immunohistochemistry: Caspase-9 Antibody [NBP1-76961] - Staining of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 ug/ml.
Western Blot: Caspase-9 Antibody [NBP1-76961] - Western blot analysis of Jurkat (A) and HeLa (B) cell lysate using Caspase 9 (NBP1-76961) antibody at 2ug/ml.
Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Staining of K562 cells using Caspase-9 antibody at 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Immunofluorescence of Caspase-9 in K562 cells with Caspase-9 antibody at 20 ug/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Caspase-9 Antibody Summary

Immunogen
Antibody was raised against a 20 amino acid peptide near the center of human Caspase-9. The immunogen is located within amino acids 290 - 340 of Caspase-9.
Specificity
Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1:1000 to 1:2000 dilution. A 46 kDa band should be detected. It has no cross response to other members in caspase family.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Caspase-9 Peptide (NBP1-76961PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caspase-9 Antibody

  • APAF-3APAF3
  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • Cas9
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • Caspase-9
  • EC 3.4.22.62
  • ICE-LAP6CASPASE-9c
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-9 Antibody (NBP1-76961). (Showing 1 - 1 of 1 FAQ).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Caspase-9 Antibody (NBP1-76961)

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Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Bioinformatics

Gene Symbol CASP9
Entrez
Uniprot