Caspase-9 [p Ser196] Antibody


Western Blot: Caspase-9 [p Ser196] Antibody [NB100-56122] - Analysis of human Jurkat cells were left untreated or were staurosporine-treated, lysed and probed with Caspase- 9 antibody at 1:1000.

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Caspase-9 [p Ser196] Antibody Summary

A synthetic phosphopeptide corresponding to amino acids 188-206 (EKLRRRFSS*LHFMVEVKGD) of human caspase-9 was used as immunogen; GenBank no. gi|27802691|gb|AAO21133.1|. S* refers to phosphorylated serine.
p Ser196
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide


  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200

Reactivity Notes

Cross reacts with Human.

Alternate Names for Caspase-9 [p Ser196] Antibody

  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • Cas9
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • caspase-9
  • EC
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease


Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Caspase-9 polyclonal antisera recognizes the proform of caspase-9 (approx. 50 kDa), and the large (approx. 35 kDa) subunit of active/cleaved Caspase-9. It does not recognize the small (approx. 15 kDa) subunit of active/cleaved Caspase-9.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu
Applications: WB, Simple Western, IP
Species: Hu, Mu, Rt, Ca, Ge
Applications: WB, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF
Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu
Applications: WB, ICC/IF, IHC-P
Supplier Logo

Publications for Caspase-9 Antibody (NB100-56122) (0)

There are no publications for Caspase-9 Antibody (NB100-56122).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Caspase-9 Antibody (NB100-56122) (0)

There are no reviews for Caspase-9 Antibody (NB100-56122). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Caspase-9 Antibody (NB100-56122). (Showing 1 - 1 of 1 FAQ).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Secondary Antibodies


Isotype Controls

Additional Caspase-9 [p Ser196] Antibody Products

Related Products by Gene

Bioinformatics Tool for Caspase-9 Antibody (NB100-56122)

Discover related pathways, diseases and genes to Caspase-9 Antibody (NB100-56122). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Caspase-9 Antibody (NB100-56122)

Discover more about diseases related to Caspase-9 Antibody (NB100-56122).

Pathways for Caspase-9 Antibody (NB100-56122)

View related products by pathway.

PTMs for Caspase-9 Antibody (NB100-56122)

Learn more about PTMs related to Caspase-9 Antibody (NB100-56122).

Research Areas for Caspase-9 Antibody (NB100-56122)

Find related products by research area.

Blogs on Caspase-9.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CASP9

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-56122 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought