APAF-1 Antibody (94408)

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 2 x 105 cells (lane 1) and 7 x 104 cells (lane 2). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human APAF‑1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
94408
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

APAF-1 Antibody (94408) Summary

Immunogen
E. coli-derived recombinant human APAF-1
Leu10-Asp214
Accession # AAC51678
Specificity
Detects human APAF-1 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APAF1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB868 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APAF-1 Antibody (94408)

  • APAF1
  • APAF-1
  • APAF-1apoptotic protease activating factor 1
  • apoptotic peptidase activating factor 1
  • apoptotic peptidase activating factor
  • apoptotic protease activating factor
  • apoptotic protease-activating factor 1
  • CED4
  • DKFZp781B1145
  • KIAA0413

Background

Apoptosis protease-activating factor 1 (APAF‑1) is a ubiquitous 135 kDa protein component of the cytoplasmic apoptosome complex. APAF‑1 plays an important role in the mitochondrial apoptotic pathway by triggering the Cytochrome c-dependent activation of Caspase-9. APAF‑1 participates in tissue morphogenesis during development, and loss of APAF‑1 expression is associated with tumor progression in a variety of cancers. Some alternate splice forms of APAF‑1 are deficient in their ability to promote apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56118
Species: Ca, Ma, Hu, Mu, Rt
Applications: Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB100-56503
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
NB100-56708
Species: Ch, ChHa, Hu, Ma, Mu, Po, Rt
Applications: CyTOF-ready, EM, Flow, HEStain, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, KD, KO, Simple Western, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NB100-56104
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
NBP1-28566
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
NB100-56116
Species: Ma, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF8221
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
NB500-218
Species: Hu, Mu
Applications: WB
NB100-56311
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
DFS00
Species: Hu
Applications: ELISA
AF860
Species: Hu, Mu
Applications: IP, Simple Western, WB
AF1457
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP2-27335
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MAB868
Species: Hu
Applications: WB

Publications for APAF-1 Antibody (MAB868)(8)

We have publications tested in 3 confirmed species: Human, Rat, Chicken.

We have publications tested in 2 applications: ICC, Western Blot.


Filter By Application
ICC
(1)
Western Blot
(7)
All Applications
Filter By Species
Human
(5)
Rat
(2)
Chicken
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB868 Applications Species
AJ Rodrigues, JJ Bandolik, FK Hansen, T Kurz, A Hamacher, MU Kassack Priming with HDAC Inhibitors Sensitizes Ovarian Cancer Cells to Treatment with Cisplatin and HSP90 Inhibitors Int J Mol Sci, 2020;21(21):. 2020 [PMID: 33167494] (Western Blot, Human) Western Blot Human
HL Hayes, BS Peterson, JM Haldeman, CB Newgard, HE Hohmeier, SB Stephens Delayed apoptosis allows islet ?-cells to implement an autophagic mechanism to promote cell survival PLoS ONE, 2017;12(2):e0172567. 2017 [PMID: 28212395] (Western Blot, Rat) Western Blot Rat
Mungamuri S, Wang S, Manfredi J, Gu W, Aaronson S Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters. Oncogene, 2015;34(19):2461-70. 2015 [PMID: 25023704] (Western Blot, Human) Western Blot Human
Kracikova , M, Akiri , G, George , A, Sachidanandam , R, Aaronson , S A A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ, 2013;20(4):576-88. 2013 [PMID: 23306555] (Western Blot, Human) Western Blot Human
Bano D, Dinsdale D, Cabrera-Socorro A, Maida S, Lambacher N, McColl B, Ferrando-May E, Hengartner MO, Nicotera P Alteration of the nuclear pore complex in Ca(2+)-mediated cell death. Cell Death Differ., 2010;17(1):119-33. 2010 [PMID: 19713973] (ICC, Rat) ICC Rat
Karki P, Seong C, Kim JE, Hur K, Shin SY, Lee JS, Cho B, Park IS Intracellular K(+) inhibits apoptosis by suppressing the Apaf-1 apoptosome formation and subsequent downstream pathways but not cytochrome c release. Cell Death Differ., 2007;14(12):2068-75. 2007 [PMID: 17885667] (Western Blot, Human) Western Blot Human
Sato T, Machida T, Takahashi S, Iyama S, Sato Y, Kuribayashi K, Takada K, Oku T, Kawano Y, Okamoto T, Takimoto R, Matsunaga T, Takayama T, Takahashi M, Kato J, Niitsu Y Fas-mediated apoptosome formation is dependent on reactive oxygen species derived from mitochondrial permeability transition in Jurkat cells. J. Immunol., 2004;173(1):285-96. 2004 [PMID: 15210786] (Western Blot, Human) Western Blot Human
Sanders EJ, Parker E The role of mitochondria, cytochrome c and caspase-9 in embryonic lens fibre cell denucleation. J. Anat., 2002;201(2):121-35. 2002 [PMID: 12220121] (Western Blot, Chicken) Western Blot Chicken

Reviews for APAF-1 Antibody (MAB868) (0)

There are no reviews for APAF-1 Antibody (MAB868). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for APAF-1 Antibody (MAB868) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional APAF-1 Products

Bioinformatics Tool for APAF-1 Antibody (MAB868)

Discover related pathways, diseases and genes to APAF-1 Antibody (MAB868). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for APAF-1 Antibody (MAB868)

Discover more about diseases related to APAF-1 Antibody (MAB868).
 

Pathways for APAF-1 Antibody (MAB868)

View related products by pathway.

PTMs for APAF-1 Antibody (MAB868)

Learn more about PTMs related to APAF-1 Antibody (MAB868).

Blogs on APAF-1.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Role of the Caspase 3 Antibody in Apoptosis Research
The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our APAF-1 Antibody (94408) and receive a gift card or discount.

Bioinformatics

Gene Symbol APAF1
Uniprot