APAF-1 Antibody (94408)

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 2 x 105 cells (lane 1) and 7 x 104 cells (lane 2). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human APAF‑1 Monoclonal Antibody (Catalog # MAB868) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for APAF‑1 was detected at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

• Reactivity: Hu
• Applications: WB
• Clone: 94408
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

APAF-1 Antibody (94408) Summary

• Immunogen: E. coli-derived recombinant human APAF-1
  Leu10-Asp214
  Accession # AAC51678
• Specificity: Detects human APAF-1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: APAF1
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APAF-1 Antibody (94408)

• APAF1
• APAF-1
• APAF-1apoptotic protease activating factor 1
• apoptotic peptidase activating factor 1
• apoptotic peptidase activating factor
• apoptotic protease activating factor
• apoptotic protease-activating factor 1
• CED4
• DKFZp781B1145
• KIAA0413

Background

Apoptosis protease-activating factor 1 (APAF‑1) is a ubiquitous 135 kDa protein component of the cytoplasmic apoptosome complex. APAF‑1 plays an important role in the mitochondrial apoptotic pathway by triggering the Cytochrome c-dependent activation of Caspase-9. APAF‑1 participates in tissue morphogenesis during development, and loss of APAF‑1 expression is associated with tumor progression in a variety of cancers. Some alternate splice forms of APAF‑1 are deficient in their ability to promote apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APAF-1 Antibody (MAB868)(8)

Publications using MAB868

• AJ Rodrigues, JJ Bandolik, FK Hansen, T Kurz, A Hamacher, MU Kassack Priming with HDAC Inhibitors Sensitizes Ovarian Cancer Cells to Treatment with Cisplatin and HSP90 Inhibitors Int J Mol Sci, 2020;21(21):. 2020 [PMID: 33167494] (Western Blot, Human)
• HL Hayes, BS Peterson, JM Haldeman, CB Newgard, HE Hohmeier, SB Stephens Delayed apoptosis allows islet ?-cells to implement an autophagic mechanism to promote cell survival PLoS ONE, 2017;12(2):e0172567. 2017 [PMID: 28212395] (Western Blot, Rat)
• Mungamuri S, Wang S, Manfredi J, Gu W, Aaronson S Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters. Oncogene, 2015;34(19):2461-70. 2015 [PMID: 25023704] (Western Blot, Human)
• Kracikova , M, Akiri , G, George , A, Sachidanandam , R, Aaronson , S A A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ, 2013;20(4):576-88. 2013 [PMID: 23306555] (Western Blot, Human)
• Bano D, Dinsdale D, Cabrera-Socorro A, Maida S, Lambacher N, McColl B, Ferrando-May E, Hengartner MO, Nicotera P Alteration of the nuclear pore complex in Ca(2+)-mediated cell death. Cell Death Differ., 2010;17(1):119-33. 2010 [PMID: 19713973] (ICC, Rat)
• Karki P, Seong C, Kim JE, Hur K, Shin SY, Lee JS, Cho B, Park IS Intracellular K(+) inhibits apoptosis by suppressing the Apaf-1 apoptosome formation and subsequent downstream pathways but not cytochrome c release. Cell Death Differ., 2007;14(12):2068-75. 2007 [PMID: 17885667] (Western Blot, Human)
• Sato T, Machida T, Takahashi S, Iyama S, Sato Y, Kuribayashi K, Takada K, Oku T, Kawano Y, Okamoto T, Takimoto R, Matsunaga T, Takayama T, Takahashi M, Kato J, Niitsu Y Fas-mediated apoptosome formation is dependent on reactive oxygen species derived from mitochondrial permeability transition in Jurkat cells. J. Immunol., 2004;173(1):285-96. 2004 [PMID: 15210786] (Western Blot, Human)
• Sanders EJ, Parker E The role of mitochondria, cytochrome c and caspase-9 in embryonic lens fibre cell denucleation. J. Anat., 2002;201(2):121-35. 2002 [PMID: 12220121] (Western Blot, Chicken)

Bioinformatics

• Gene Symbol: APAF1
• Uniprot:
  • AAC51678()