APAF-1 Antibody (94408)


Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 2 x 105cells (lane 1) and 7 x 104cells (lane 2). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human APAF-1 ...read more

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Reactivity HuSpecies Glossary
Applications WB

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APAF-1 Antibody (94408) Summary

E. coli-derived recombinant human APAF-1
Accession # AAC51678
Detects human APAF-1 in Western blots.
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
Read Publications using
MAB868 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APAF-1 Antibody (94408)

  • APAF1
  • APAF-1
  • APAF-1apoptotic protease activating factor 1
  • apoptotic peptidase activating factor 1
  • apoptotic peptidase activating factor
  • apoptotic protease activating factor
  • apoptotic protease-activating factor 1
  • CED4
  • DKFZp781B1145
  • KIAA0413


Apoptosis protease-activating factor 1 (APAF‑1) is a ubiquitous 135 kDa protein component of the cytoplasmic apoptosome complex. APAF‑1 plays an important role in the mitochondrial apoptotic pathway by triggering the Cytochrome c-dependent activation of Caspase-9. APAF‑1 participates in tissue morphogenesis during development, and loss of APAF‑1 expression is associated with tumor progression in a variety of cancers. Some alternate splice forms of APAF‑1 are deficient in their ability to promote apoptosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APAF-1 Antibody (MAB868)(8)

We have publications tested in 3 confirmed species: Human, Rat, Chicken.

We have publications tested in 2 applications: ICC, Western Blot.

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Showing Publications 1 - 8 of 8.
Publications using MAB868 Applications Species
AJ Rodrigues, JJ Bandolik, FK Hansen, T Kurz, A Hamacher, MU Kassack Priming with HDAC Inhibitors Sensitizes Ovarian Cancer Cells to Treatment with Cisplatin and HSP90 Inhibitors Int J Mol Sci, 2020-11-05;21(21):. 2020-11-05 [PMID: 33167494] (Western Blot, Human) Western Blot Human
HL Hayes, BS Peterson, JM Haldeman, CB Newgard, HE Hohmeier, SB Stephens Delayed apoptosis allows islet ?-cells to implement an autophagic mechanism to promote cell survival PLoS ONE, 2017-02-17;12(2):e0172567. 2017-02-17 [PMID: 28212395] (Western Blot, Rat) Western Blot Rat
Mungamuri S, Wang S, Manfredi J, Gu W, Aaronson S Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters. Oncogene, 2014-07-14;34(19):2461-70. 2014-07-14 [PMID: 25023704] (Western Blot, Human) Western Blot Human
Kracikova , M, Akiri , G, George , A, Sachidanandam , R, Aaronson , S A A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ, 2013-01-11;20(4):576-88. 2013-01-11 [PMID: 23306555] (Western Blot, Human) Western Blot Human
Bano D, Dinsdale D, Cabrera-Socorro A, Maida S, Lambacher N, McColl B, Ferrando-May E, Hengartner MO, Nicotera P Alteration of the nuclear pore complex in Ca(2+)-mediated cell death. Cell Death Differ., 2010-01-01;17(1):119-33. 2010-01-01 [PMID: 19713973] (ICC, Rat) ICC Rat
Karki P, Seong C, Kim JE, Hur K, Shin SY, Lee JS, Cho B, Park IS Intracellular K(+) inhibits apoptosis by suppressing the Apaf-1 apoptosome formation and subsequent downstream pathways but not cytochrome c release. Cell Death Differ., 2007-09-21;14(12):2068-75. 2007-09-21 [PMID: 17885667] (Western Blot, Human) Western Blot Human
Sato T, Machida T, Takahashi S, Iyama S, Sato Y, Kuribayashi K, Takada K, Oku T, Kawano Y, Okamoto T, Takimoto R, Matsunaga T, Takayama T, Takahashi M, Kato J, Niitsu Y Fas-mediated apoptosome formation is dependent on reactive oxygen species derived from mitochondrial permeability transition in Jurkat cells. J. Immunol., 2004-07-01;173(1):285-96. 2004-07-01 [PMID: 15210786] (Western Blot, Human) Western Blot Human
Sanders EJ, Parker E The role of mitochondria, cytochrome c and caspase-9 in embryonic lens fibre cell denucleation. J. Anat., 2002-08-01;201(2):121-35. 2002-08-01 [PMID: 12220121] (Western Blot, Chicken) Western Blot Chicken

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Secondary Antibodies


Isotype Controls

Additional APAF-1 Products

Blogs on APAF-1.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Role of the Caspase 3 Antibody in Apoptosis Research
The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are...  Read full blog post.

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Gene Symbol APAF1