Caspase-9 Antibody (LAP6 96-2-22)

Images

 
Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - Western blot analysis of Jurkat (A) and HeLa (B) cell lysate using Caspase 9 (NB500-209) antibody at 1:500.
Immunocytochemistry/ Immunofluorescence: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - The Caspase 9 antibody was tested in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more
Immunohistochemistry-Paraffin: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - IHC analysis of paraffin-embedded human breast carcinomausing Caspase 9 antibody at 1:25. Image from verified customer review.
Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). Lane 1: inactive 293 cell lysate.
Simple Western: Caspase-9 Antibody (LAP6 96-2-22) [NB500-209] - Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Staurosporine (STS) for 3 hours, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, KD
Clone
LAP6 96-2-22
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Caspase-9 Antibody (LAP6 96-2-22) Summary

Immunogen
Recombinant human Caspase 9 pro-domain protein. [UniProt# P55211]
Localization
Cytoplasmic and Mitochondrial
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP9
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:250
  • Knockdown Validated
Application Notes
This Caspase 9 (LAP6 96-2-22) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where bands at ~46kDa-48kDa and 35kDa are seen representing the pro and active forms of Caspase 9, respectively. In ICC/IF cytoplasmic staining can be seen in HeLa cells. Paraffin data from customer review.
Control
293 Lysate (NB800-PC6)
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB500-209 in the following applications:

Publications
Read Publications using
NB500-209 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified

Notes

Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Staurosporine (STS) for 3 hours, loaded at 0.2 mg/mL. A specific band was detected for Caspase 9 at approximately 53 kDa (as indicated) using 20 ug/mL of Mouse Anti-Human Caspase 9 Monoclonal Antibody (Catalog # MAB8301). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Alternate Names for Caspase-9 Antibody (LAP6 96-2-22)

  • APAF-3APAF3
  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • Caspase-9
  • EC 3.4.22.62
  • ICE-LAP6CASPASE-9c
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease

Background

Among the initiator caspases (caspases -2, -8 and -9), caspase-9 is an essential enzymes required for intrinsic or mitochondrial apoptotic pathway. Caspase 9 is a heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kD (p35) and a 10 kD (p10) subunit, and it interacts with several proteins including APAF1, EFHD2, HAX1, BIRC2/c-IAP1, XIAP/BIRC4, BIRC5/survivin, BIRC6/bruce, BIRC7/livin etc. During apoptosome-dependent apoptotic execution, cytochrome-c is released from the mitochondria into cytosol where it binds/activates Apaf-1. Upon nucleotide exchange in the presence of dATP, Apaf-1 oligomerizes into heptameric apoptosome complex and procaspase-9 is recruited to apoptosome where it gets activated. Active caspase-9 at the apoptosome proteolytically activates procaspase-3 (the critical apoptotic executioner caspases) and cleaves poly(ADP-ribose) polymerase (PARP). Both caspases-9 and -3 can be inhibited by selected members of IAPs, with XIAP being their most potent natural inhibitor. Phosphorylation of a major site, Thr125 by CDK1-cyclin B1 leading to inhibition of caspase 9 plays a critical role in the restraint of apoptosis during mitotic arrest. This site is also targeted by ERK1/2 in response to growth factor signalling and by DYRK1A, which plays important roles during development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-9 Antibody (NB500-209)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(4)
All Species

Reviews for Caspase-9 Antibody (NB500-209) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using NB500-209:
Filter by Applications
IHC-P
(1)
WB
(3)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Caspase-9 NB500-209
Enlarge
reviewed by:
Anony- mous
IHC-P Human 08/29/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
SpeciesHuman
Special ApplicationsThis antibody detected endogenous level of Caspase 9 in human breast carcinoma in IHC at dilution of 1:25.
FileView PDF
Western Blot Caspase-9 NB500-209
Enlarge
reviewed by:
Anony- mous
WB Human 08/29/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsThis antibody detected endogenous level of Caspase 9 in C6 cell lysates in WB at dilution of 1:1000
FileView PDF
Western Blot Caspase-9 NB500-209
Enlarge
reviewed by:
Anonymous
WB Human 01/13/2012
View

Summary

ApplicationWestern Blot
Sample TestedCOLO-205 Cell Line
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat dry milk in TBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti Mouse HRP, Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL, Specific Bands: 35 kDa
 
reviewed by:
Anonymous
WB Human 12/23/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman cell
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in TBST Buffer

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 1 hour

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-mouse IgG, HRP conjugated

Details

Detection NotesDetection Method: ECL

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-9 Antibody (NB500-209). (Showing 1 - 2 of 2 FAQs).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.
  2. Does NB500-209 both recognize human and rat active form of caspase 9 (subunit p35 or subunit p10)? Besides that please let me know if NB500-209 also recognize pro-form caspase 9?
    • Caspase 9 Antibody (LAP6 96-2-22) # NB500-209 is human specific and do not recognize rat samples. It is expected to detect pro as well as activated form of caspase 9. In our QC data using 293 cell lysates, we observed active subunit p35 only not subunit p10. Both of these antibodies were tested on lysates of 293 cells of human origin (also called Human Embryonic Kidney 293 cells). We did not validate them on rat samples as the antibodies are Human specific.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Caspase-9.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Anony- mous
08/29/2014
Application: IHC-P
Species: Human

Anony- mous
08/29/2014
Application: WB
Species: Human

Anonymous
01/13/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol CASP9
Entrez
Uniprot