Caspase-8 Antibody (90A992) [Biotin]

Western Blot: Caspase-8 Antibody (90A992) [Biotin] [NB100-55786] - Analysis for human Caspase-8 using Jurkat lysates with NB100-55786 at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2) dilution. NB100-55786 only detects 55 kDa Caspase-8 in Jurkat cells.

• Reactivity: Hu, Pm
• Applications: WB, ELISA
• Clone: 90A992
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

Caspase-8 Antibody (90A992) [Biotin] Summary

• Immunogen: The immunogen for this product falls between amino acids 360-385 of the human Caspase 8 protein.
• Specificity: This can be used for detection of the pro-form of Caspase-8.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CASP8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.5-2 ug/ml
  • Western Blot 0.5-2 ug/ml
• Application Notes: Optimal dilution of this antibody should be experimentally determined.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371
• Control:

  Jurkat Whole Cell Lysate
  Caspase-8 Overexpression Lysate
  Jurkat Staurosporine Treated / Untreated Cell Lysate

Reactivity Notes

Chimpanzee and Rhesus Monkey reactivity noted.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Caspase-8 Antibody (90A992) [Biotin]

• AIS
• androgen receptor
• CASP8
• Caspase8
• Caspase-8
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• Mch5
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-8 (FLICE) forms a direct link between the activation of CD95 and the caspase pathway. Overexpression of Caspase-8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-8 Antibody (NB100-55786)(1)

Publication using NB100-55786

• Schultz CR, Golembieski WA, King DA et al. Inhibition of HSP27 alone or in combination with pAKT inhibition as therapeutic approaches to target SPARC-induced glioma cell survival. Mol Cancer. 2012-04-05 [PMID: 22480225]

FAQs for Caspase-8 Antibody (NB100-55786). (Showing 1 - 1 of 1 FAQ).

1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
   • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Bioinformatics

• Gene Symbol: CASP8