Caspase-3 Antibody - (active/cleaved)

Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) - Unpurified [NB100-56113] - Irradiated mouse spleen stained for Active/Cleaved Caspase-3 expression using NB100-56113 at 1:2000. Staining is seen in the nuclei of a subset of the cell population. Caspase-3 expression in the nucleus is considered to be a marker of active/caspase-3 expression and apoptosis. Hematoxylin-eosin counterstain.

Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) - Unpurified [NB100-56113] - Dog ischemic brain stained for Active/Cleaved Caspase-3 expression using NB100-56113 at 1:2000. Staining is seen in the nuclei of dying neurons (black arrow) but not in the morphologically normal nuclei (open arrows). Caspase-3 expression in the nucleus is considered to be a marker of active/caspase-3 expression and apoptosis. Hematoxylin-eosin counterstain.

• Reactivity: Hu, Mu, Rt, Ge
• Applications: WB, Flow, Flow-IC, IB, IHC, IHC-Fr, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Caspase-3 Antibody - (active/cleaved) Summary

• Immunogen: Recombinant catalytically active human caspase-3 protein was used as immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 - 1:2000
  • Flow Cytometry
  • Immunoblotting
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 1:50 - 1:200
  • Flow (Intracellular)
• Application Notes: Preferentially detects active caspase-3 (large subunit: ~14-21 kDa, and small subunit: ~10 kDa). However, it may also detect pro-caspase-3 (~32 kDa) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3. Use in Flow Intracellular reported in scientific literature (PMID 24804954). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29864441). Use in FLOW reported in scientific literature (PMID: 28287161). Use in immunoblotting reported in scientific literature (PMID: 27912032).
• Control:

  Caspase-3 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Neat whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Caspase-3 Antibody - (active/cleaved)

• Apopain
• apoptosis-related cysteine protease
• CASP3
• CASP-3
• caspase 3, apoptosis-related cysteine peptidase
• Caspase3
• Caspase-3
• CPP32
• CPP-32
• CPP32B
• CPP32SREBP cleavage activity 1
• Cysteine protease CPP32
• EC 3.4.22
• EC 3.4.22.56
• LICE-1
• PARP cleavage protease
• procaspase3
• Protein Yama
• SCA-1
• YAMA

Background

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p20-p10. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Active/Cleaved Caspase-3 polyclonal antisera recognizes the large (~14-21 kDa) and small (~10 kDa) subunits of active/cleaved caspase-3. Whereas the antisera has a strong preference for active/cleaved caspase-3, in some cell or tissue systems or techniques the antisera may also recognize the proform of caspase-3 (~32 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-3 Antibody (NB100-56113)(29)

Publications using NB100-56113

• Shaalan A, Proctor G Salivary glands require Aurora Kinase B for regeneration after transient innate immune-mediated injury Sci Rep Aug 5 2019 [PMID: 31383943] (WB, IHC-P, Mouse)
• Solek P, Koszla O, Mytych J et al. Neuronal life or death linked to depression treatment: the interplay between drugs and their stress-related outcomes relate to single or combined drug therapies Apoptosis Jul 5 2019 [PMID: 31278507] (WB, Mouse)
• Stojanovic B, Milovanovic J, Arsenijevic A et al. Galectin-3 Deficiency Facilitates TNF-a-Dependent Hepatocyte Death and Liver Inflammation in MCMV Infection Front Microbiol Feb 8 2019 [PMID: 30800112] (IHC-P, Mouse)
• Codenotti S, Faggi F, Ronca R et al. Caveolin-1 enhances metastasis formation in a human model of embryonal rhabdomyosarcoma through Erk signaling cooperation Cancer Lett. Feb 13 2019 [PMID: 30771426] (WB, Human)
• Corsetti G, Chen-Scarabelli C, Romano C et al. Autophagy and Oncosis/Necroptosis Are Enhanced in Cardiomyocytes from Heart Failure Patients Med Sci Monit Basic Res Feb 4 2019 [PMID: 30713336] (IHC-P, Human)
• Hu S, Cheng M, Guo X et al. Down-regulation of miR-200c attenuates AngII-induced cardiac hypertrophy via targeting the MLCK-mediated pathway. J. Cell. Mol. Med. Jan 25 2019 [PMID: 30680929] (WB, Rat)
• Dahiya NR, Chandrasekaran B, Kolluru V et al. A natural molecule, urolithin A, downregulates androgen receptor activation and suppresses growth of prostate cancer Mol. Carcinog. Aug 1 2018 [PMID: 30069922] (WB, Human)
• Solek P, Majchrowicz L, Koziorowski M. Aloe arborescens juice prevents EMF-induced oxidative stress and thus protects from pathophysiology in the male reproductive system in vitro Environ. Res. Jun 7 2018 [PMID: 29886390] (Mouse)
• Talreja D, Cashman SM, Dasari B et al. G-quartet oligonucleotide mediated delivery of functional X-linked inhibitor of apoptosis protein into retinal cells following intravitreal injection Exp. Eye Res. Jun 1 2018 [PMID: 29864441] (IHC-Fr, Mouse)
• Guo Y, Wang F, Li H et al. Metformin Protects Against Spinal Cord Injury by Regulating Autophagy via the mTOR Signaling Pathway Neurochem. Res. May 4 2018 [PMID: 29728793] (Rat)
• Adamson Samantha E, Polanowska-Grabowska Renata, Marqueen Kathryn et al. Deficiency of Dab2 (Disabled Homolog 2) in Myeloid Cells Exacerbates Inflammation in Liver and Atherosclerotic Plaques in LDLR (Low-Density Lipoprotein Receptor)-Null Mice-Brief Report. Arteriosclerosis, Thrombosis, and Vascular Biology 2018 Mar 29 [PMID: 29599136] (IHC, Mouse)
• Shaalan A, Carpenter G, Proctor G. Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo. Mol Immunol 2018 Feb [PMID: 29331803] (Mouse)
• Bozec A, Zangari J, Butori-Pepino M, Ilie M et al. MiR-223-3p inhibits angiogenesis and promotes resistance to cetuximab in head and neck squamous cell carcinoma Oncotarget 2017 Aug 22 [PMID: 28915663] (IHC, Human)
• Mytych J, Romerowicz-Misielak M, Koziorowski M. Long-term culture with lipopolysaccharide induces dose-dependent cytostatic and cytotoxic effects in THP-1 monocytes. Toxicol In Vitro. Mar 21 2017 [PMID: 28341289]
• Solek P, Majchrowicz L, Bloniarz D et al. Pulsed or continuous electromagnetic field induce p53/p21-mediated apoptotic signaling pathway in mouse spermatogenic cells in vitro and thus may affect male fertility. Toxicology. Mar 16 2017 [PMID: 28323003] (WB, Mouse)
• Phua T, Sng MK, Tan EH et al. Angiopoietin-like 4 Mediates Colonic Inflammation by Regulating Chemokine Transcript Stability via Tristetraprolin. Sci Rep. Mar 13 2017 [PMID: 28287161] (Flow, Mouse)
• Sidhar H, Giri RK. Induction of Bex genes by curcumin is associated with apoptosis and activation of p53 in N2a neuroblastoma cells. Sci Rep. Feb 1 2017 [PMID: 28145533] (WB, Mouse)
• Rasheed Nora O Abdel, Ahmed Lamiaa A, Abdallah Dalaal M, El-Sayeh Bahia M Nephro-toxic effects of intraperitoneally injected EGCG in diabetic mice: involvement of oxidative stress, inflammation and apoptosis. Scientific Reports 2017 Jan 18 [PMID: 28098182] (IHC-P, Mouse)
• Corsetti G, Romano C, Stacchiotti A et al. Endoplasmic Reticulum Stress and Apoptosis Triggered by Sub-Chronic Lead Exposure in Mice Spleen: a Histopathological Study. Biol Trace Elem Res. Dec 24 2016 [PMID: 28012149] (Mouse)
• Mytych J, Wos I, Solek P, Koziorowski M. Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes. Exp Cell Res 2017 Jan 15 [PMID: 28011195] (Human)
• Turtle JD, Strain MM, Aceves M et al. Pain Input Impairs Recovery after Spinal Cord Injury: Treatment with Lidocaine. J Neurotrauma 2017 Mar 15 [PMID: 27912032]
• Li T, Sun KJ, Wang HD et al. Tert-butylhydroquinone Ameliorates Early Brain Injury After Experimental Subarachnoid Hemorrhage in Mice by Enhancing Nrf2-Independent Autophagy. Neurochem. Res. 2015 Sep 01 [PMID: 26260377] (WB, Mouse)
• Garraway SM, Woller SA, Huie JR et al. Peripheral noxious stimulation reduces withdrawal threshold to mechanical stimuli after spinal cord injury: Role of tumor necrosis factor alpha and apoptosis. Pain. 2014 Aug 29 [PMID: 25180012]
• Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014 May 07 [PMID: 24804954] (Flow-IC, Human)
  
  Details:
  Jurkat, Fig 9.
• Aulakh Gurpreet K, Balachandran Yadu, Liu Lixin, Singh Baljit. Angiostatin inhibits activation and migration of neutrophils. Cell Tissue Res. 2014 Feb [PMID: 24297047]
• Tsai AC, Pai HC, Wang CY et al. In vitro and in vivo anti-tumour effects of MPT0B014, a novel derivative aroylquinoline, and in combination with erlotinib in human non-small-cell lung cancer cells. Br J Pharmacol 2014 Jan [PMID: 24116948] (WB, Human)
• Liu G, Keeler BE, Zhukareva V, Houle JD. Cycling exercise affects the expression of apoptosis-associated microRNAs after spinal cord injury in rats. Exp Neurol. 2010 Nov [PMID: 20816819] (WB, Rat)
  
  Details:
  Caspase-3 Active/Cleaved (IMG-5700), Caspase-9 Active/Cleaved (IMG-5705). WB: Rat spinal cord tissue ten days after the animal sustained spinal cord injury, Fig 7A.Note: Caspase-3 pAb was used at 1:2500 and Caspase-9 pAb at 1:3000 for WB, Fig 7A.
• Matsuda N, Teramae H, Futatsugi M et al. Up-regulation of histamine H4 receptors contributes to splenic apoptosis in septic mice: counteraction of the antiapoptotic action of nuclear factor-kappaB J Pharmacol Exp Ther 2010 Mar [PMID: 20008488] (WB, Mouse)
• Sato K, Arai H, Miyazawa Y et al. Palatinose and oleic acid act together to prevent pancreatic islet disruption in nondiabetic obese Zucker rats. J Med Invest. 2008 Aug [PMID: 18797130] (IHC-P)
  
  Details:
  Caspase-3 Active/Cleaved (IMG-5700). IF/ICC: Pancreatic islet cells from Zucker rats, the animals were fed a diet of carbohydrates and fats, Fig 5.

FAQs for Caspase-3 Antibody (NB100-56113). (Showing 1 - 1 of 1 FAQs).

1. I used your NB100-56113 and I was wondering if the positive signal could show up in the cytoplasm
   • The caspase 3 protein is a cytoplasmic protein that can be transported from the cytoplasm to the nucleus during apoptosis, so it is possible that the antibody can be found in both locations.

Bioinformatics

• Gene Symbol: CASP3