Caspase-3 Antibody - (active/cleaved)


Biological Strategies: Immunohistochemistry: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Immunohistochemistry analysis of sectioned tumor tissues from the MDA-MB-231 study. Each section was subjected to more
Biological Strategies: Western Blot: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - The protein expression levels of Bcl-2, Bax and cleaved caspase-3 were detected in the different groups using Western more
Western Blot: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Cell apoptosis was detected by flow cytometric analysis, and the percentage of apoptotic cells transfected with miR-200c inhibitor or NC inhibitor and more
Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Dog ischemic brain stained for Active/Cleaved Caspase-3 expression using Caspase-3 Antibody - (active/cleaved)( NB100-56113) at more
Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Irradiated mouse spleen stained for Active/Cleaved Caspase-3 expression using Caspase-3 Antibody - (active/cleaved) (NB100-56113) at more

Product Details

Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Validated by:

Biological Strategies


Order Details

Caspase-3 Antibody - (active/cleaved) Summary

This Caspase-3 Antibody - (active/cleaved) was developed against catalytically active human caspase-3 protein.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow (Intracellular)
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
Application Notes
Preferentially detects active caspase-3 (large subunit: ~14-21 kDa, and small subunit: ~10 kDa). However, it may also detect pro-caspase-3 (~32 kDa) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3. Use in Flow Intracellular reported in scientific literature (PMID 24804954). Use in IHC-F reported in scientific literature (PMID: 29864441). Use in FLOW reported in scientific literature (PMID: 28287161). Use in immunoblotting reported in scientific literature (PMID: 27912032). Use in ICC/IF was reported in scientific literature (PMID: 29963272).
Theoretical MW
31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Jurkat Staurosporine Treated / Untreated Cell Lysate
Read Publications using
NB100-56113 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34597692). Rat reactivity reported in scientific literature (PMID:32818590).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Neat whole antisera
0.05% Sodium Azide

Alternate Names for Caspase-3 Antibody - (active/cleaved)

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA


CPP32 encodes a member of the cysteine-aspartic acid protease (caspase) family that plays a key role in apoptosis. Caspase 3 (also known as CPP32, Apopain, Yama, and PARP cleavage protease) is the most extensively studied apoptotic protein among caspase family members (1-2). Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). Alternative splicing of CPP32 results in two transcript variants which encode the same protein. The processed form of Caspase 3 consists of large (theoretical molecular weight 17kD) and small (theoretical molecular weight 12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease (3).


1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G., . . . Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3

2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y., . . . Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199

3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A., . . . Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-3 Antibody (NB100-56113)(62)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Gerbil.

We have publications tested in 7 applications: FLOW, Flow Cytometry Control, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 62. Show All 62 Publications.
Publications using NB100-56113 Applications Species
Ruiz TFR, Grigio V, Ferrato LJ et al. Impairment of steroidogenesis and follicle development after bisphenol A exposure during pregnancy and lactation in the ovaries of Mongolian gerbils aged females Molecular and cellular endocrinology 2023-02-20 [PMID: 36813021]
Elkhoely A Liraglutide ameliorates gentamicin-induced acute kidney injury in rats via PGC-1 alpha - mediated mitochondrial biogenesis: Involvement of PKA/CREB and Notch/Hes-1 signaling pathways International immunopharmacology 2022-12-14 [PMID: 36525794] (IF/IHC, Rat) IF/IHC Rat
Xu H, Ye W, Shi B LncRNA MALAT1 Regulates USP22 Expression Through EZH2-Mediated H3K27me3 Modification to Accentuate Sepsis-Induced Myocardial Dysfunction Cardiovascular toxicology 2022-06-20 [PMID: 35726125]
Kelly YM, Ward C, Zhang R et al. Effects of Multi-Stage Procurement on the Viability and Function of Human Donor Parathyroid Glands The Journal of surgical research 2022-04-22 [PMID: 35468367] (IHC-P, Human) IHC-P Human
Chen L, Xu Y, Ye X Low temperature mitigating the paclitaxel-induced damages in mouse cell and hair follicle model Biochemical and biophysical research communications 2022-05-07 [PMID: 35279463] (IF/IHC, Mouse) IF/IHC Mouse
Ma, N;Li, G;Fu, X; Protective role of activating transcription factor 3 against neuronal damage in rats with cerebral ischemia Brain and behavior [PMID: 35263513] (WB, Rat) WB Rat
Ruiz TFR, Leonel ECR, Colleta SJ et al. Gestational and lactational xenoestrogen exposure disrupts morphology and inflammatory aspects in mammary gland of gerbil mothers during involution Environmental toxicology and pharmacology 2022-01-01 [PMID: 34896274] (IHC-P, Gerbil) IHC-P Gerbil
Saha A, Saleem S, Kumar PR, Biswas SC BH3-only proteins Puma and Beclin1 regulate autophagic death in neurons in response to Amyloid-beta Cell death discovery 2021-11-15 [PMID: 34782612] (ICC/IF, Rat) ICC/IF Rat
Zhao J, Dong L, Huo T Et al. O-GlcNAc Transferase (OGT) Protects Cerebral Neurons from Death During Ischemia/Reperfusion (I/R) Injury by Modulating Drp1 in Mice Neuromolecular medicine 2021-10-27 [PMID: 34705256]
Ahmed ZSO, Galal MK, Drweesh EA et al. Protective effect of starch-stabilized selenium nanoparticles against melamine-induced hepato-renal toxicity in male albino rats International journal of biological macromolecules 2021-09-28 [PMID: 34597692] (IHC-P, Rat) IHC-P Rat
Show All 62 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-3 Antibody (NB100-56113). (Showing 1 - 1 of 1 FAQs).

  1. I used your NB100-56113 and I was wondering if the positive signal could show up in the cytoplasm
    • The caspase 3 protein is a cytoplasmic protein that can be transported from the cytoplasm to the nucleus during apoptosis, so it is possible that the antibody can be found in both locations.

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Blogs on Caspase-3. Showing 1-10 of 11 blog posts - Show all blog posts.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.

  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Cleaved Caspase-3: A Marker of Programmed Cell Death
What are Caspases?Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environme...  Read full blog post.

Caspase-3, The Executioner of Apoptosis
The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
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Gene Symbol CASP3