CART/CARTPT Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CART/CARTPT Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CART
  Gln28-Leu116
  Accession # Q16568
• Specificity: Detects human CART in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CARTPT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CART/CARTPT Antibody [Biotin]

• CART prepropeptide
• CART
• CARTcocaine and amphetamine regulated transcript
• CARTPT
• cocaine- and amphetamine-regulated transcript protein

Background

CART, also known as cocaine and amphetamine-regulated transcript, is a 10 kDa secreted polypeptide produced by neuronal cell types in a variety of locations. In human, the molecule is synthesized as a 116 amino acid (aa) precursor with a 27 aa signal sequence an 89 aa mature segment. There are six C-terminal cysteines that form three intrachain disulfide bonds, and the potential exists for both sulfation and phosphorylation in the N-terminal region. There are numerous monobasic and dibasic sites for enzyme cleavage, and multiple, presumably proteolytically-generated short forms are known to exist. These include a 5K, 48 aa peptide
(CART 42‑89), and a 4.3K, 40 aa peptide (CART 49-89). Human CART is 98% aa identical to both moyuse and rat CART (1-89). Although CART suppresses feeding and increases pain tolerance, there would appear to be differences between the various forms of CART on other functions. In addition, anatomical location is associated with differing lengths of CART. For example, 89 aa and 79 aa forms are found in the adrenal, while 48 aa and 44 aa forms are found in the hypothalamus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CART/CARTPT Antibody (BAF163)(1)

Publication using BAF163

• Ji-Yao Li, Xiaoyin Wu, Allen Lee, Shi-Yi Zhou, Chung Owyang Altered R-spondin 1/CART neurocircuit in the hypothalamus contributes to hyperphagia in diabetes Journal of Neurophysiology 2019-03-01 [PMID: 30649980]

Bioinformatics

• Gene Symbol: CARTPT
• Uniprot:
  • Q16568()