CARD12 Antibody [FITC]

Images

 
There are currently no images for CARD12 Antibody (NBP1-78979F).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

CARD12 Antibody [FITC] Summary

Immunogen
A synthetic peptide made to an internal portion of the human CARD12 protein (between residues 600-700) [UniProt Q9NPP4]
Localization
Cytoplasm. Note: Cytoplasmic filaments.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NLRC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This CARD12 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CARD12 Antibody [FITC]

  • CARD12leucine rich repeat and CARD domaincontaining 4
  • caspase recruitment domain family, member 12
  • Caspase recruitment domain-containing protein 12
  • Clan protein
  • CLAN1ipaf
  • CLANCARD, LRR, and NACHT-containing protein
  • CLR2.1
  • Ice protease-activating factor
  • Ipaf
  • NLR family, CARD domain containing 4

Background

CARD12, also known as CLAN, IPAF and CLAN1 is a member of the CED4 family of proteins. The CED4/Apaf-1 proteins are critical regulators of apoptosis and NF-kappaB signaling pathways, and CARD12 is no exception. CARD12 has been implicated in coordination of downstream activation of both apoptotic and inflammatory signaling pathways (PMID: 11374873). Additionally, CARD12 has shown to coprecipitate with caspase 1, an enzyme that participates in both the signaling of apoptosis and cytokine processing, further demonstrating its integral role in programmed cell death (PMID: 11374873). As part of the nucleotide-binding site and leucine-rich repeat (NBS-LRR) proteins, CARD12 is known to be an important regulator of caspase-1 activation via an ATP/dATP nucleotide-binding pocket (PMID: 15882992). CARD12 has four known isoforms. Isoform 1 is highly expressed in lung, followed by leukocytes (especially monocytes), lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Although it is expressed ubiquitously, Isoform 2 is more prominent at the highest levels in lung and spleen. Isoform 4 can only been found in the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-12446
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56565
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP1-78977
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-90095
Species: Hu
Applications: IHC, IHC-P
NBP1-54899
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr
H00009447-B01P
Species: Hu
Applications: WB, ELISA, ICC/IF
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
MAB829
Species: Hu
Applications: WB, IHC
NBP2-24787
Species: Hu, Mu, Ca
Applications: WB, Flow, IHC, IHC-Fr, IHC-P, Flow-CS, Flow-IC
NB100-56152
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP2-17262
Species: Hu
Applications: WB, ICC/IF
AF6567
Species: Hu, Mu
Applications: WB, IHC

Publications for CARD12 Antibody (NBP1-78979F) (0)

There are no publications for CARD12 Antibody (NBP1-78979F).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CARD12 Antibody (NBP1-78979F) (0)

There are no reviews for CARD12 Antibody (NBP1-78979F). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CARD12 Antibody (NBP1-78979F) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CARD12 Products

Bioinformatics Tool for CARD12 Antibody (NBP1-78979F)

Discover related pathways, diseases and genes to CARD12 Antibody (NBP1-78979F). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CARD12 Antibody (NBP1-78979F)

Discover more about diseases related to CARD12 Antibody (NBP1-78979F).
 

Pathways for CARD12 Antibody (NBP1-78979F)

View related products by pathway.

PTMs for CARD12 Antibody (NBP1-78979F)

Learn more about PTMs related to CARD12 Antibody (NBP1-78979F).
 

Research Areas for CARD12 Antibody (NBP1-78979F)

Find related products by research area.

Blogs on CARD12

There are no specific blogs for CARD12, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CARD12 Antibody [FITC] and receive a gift card or discount.

Bioinformatics

Gene Symbol NLRC4