| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85691-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48854-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4920-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4920-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21940-50ul | |||
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Analysis in human liver and cerebral cortex tissues using NBP1-85691 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Analysis in human liver and cerebral cortex tissues using NBP1-85691 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0026.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0021.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0025.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0027.jpg)
![Simple Western: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Simple Western lane view shows a specific band for CES1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Simple-Western-NBP1-85691-img0006.jpg)
![Simple Western: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Electropherogram image(s) of corresponding Simple Western lane view. CES1 antibody was used at 1:20 dilution on RT-4 lysate(s).](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Simple-Western-NBP1-85691-img0007.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Analysis in human liver and cerebral cortex tissues using NBP2-48854 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Analysis in human liver and cerebral cortex tissues using NBP2-48854 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human cerebral cortex, liver, prostate and upper gastrointestinal using Anti-CES1 antibody NBP2-48854 (A) shows similar protein distribution across tissues to independent antibody NBP1-85691 (B).](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Analysis of human cell line Hep G2 shows positivity in endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48854-img0001.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0017.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0013.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0015.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0016.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0018.jpg)
![Western Blot: Carboxylesterase 1/CES1 Antibody (JE33-04) [NBP3-32541] - Western blot analysis of Carboxylesterase 1/CES1 on different lysates with Rabbit anti-Carboxylesterase 1/CES1 antibody (NBP3-32541) at 1/1,000 dilution. Lane 1: U-937 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (10 ug/Lane) Lane 3: Human lung tissue lysate (10 ug/Lane) Predicted band size: 62.5 kDa Observed band size: 55 kDa Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32541) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32541_rabbit-carboxylesterase-1-ces1-mab-je33-04-278202416331252.jpg "Western Blot: Carboxylesterase 1/CES1 Antibody (JE33-04) [NBP3-32541] - Western blot analysis of Carboxylesterase 1/CES1 on different lysates with Rabbit anti-Carboxylesterase 1/CES1 antibody (NBP3-32541) at 1/1,000 dilution. Lane 1: U-937 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (10 ug/Lane) Lane 3: Human lung tissue lysate (10 ug/Lane) Predicted band size: 62.5 kDa Observed band size: 55 kDa Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32541) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Carboxylesterase 1/CES1 Antibody (JE33-04) [NBP3-32541] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Carboxylesterase 1/CES1 antibody (NBP3-32541) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32541) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32541_rabbit-carboxylesterase-1-ces1-mab-je33-04-298202414304183.jpg)
![Immunocytochemistry/ Immunofluorescence: Carboxylesterase 1/CES1 Antibody (JE33-04) [NBP3-32541] - Immunocytochemistry analysis of U-937 cells labeling Carboxylesterase 1/CES1 with Rabbit anti-Carboxylesterase 1/CES1 antibody (NBP3-32541) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Carboxylesterase 1/CES1 antibody (NBP3-32541) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32541_rabbit-carboxylesterase-1-ces1-mab-je33-04-2920241192346.jpg)
![Flow Cytometry: Carboxylesterase 1/CES1 Antibody (JE33-04) [NBP3-32541] - Flow cytometric analysis of U-937 cells labeling Carboxylesterase 1/CES1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32541, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32541_rabbit-carboxylesterase-1-ces1-mab-je33-04-39202488460.jpg)
![Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388] - Western blot analysis of extracts of various cell lines, using Carboxylesterase 1/CES1 Rabbit mAb (NBP3-15388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-7C9R6-Western-Blot-NBP3-15388-img0002.jpg "Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388] - Western blot analysis of extracts of various cell lines, using Carboxylesterase 1/CES1 Rabbit mAb (NBP3-15388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388] - Immunofluorescence analysis of HeLa cells using Carboxylesterase 1/CES1 Rabbit mAb (NBP3-15388) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-7C9R6-Immunocytochemistry-Immunofluorescence-NBP3-15388-img0001.jpg)
![Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388] - Western blot analysis of extracts of HepG2 cells, using Carboxylesterase 1/CES1 Rabbit mAb (NBP3-15388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-7C9R6-Western-Blot-NBP3-15388-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Carboxylesterase 1/CES1 Antibody (7C9R6) [Carboxylesterase 1/CES1] - Confocal imaging of paraffin-embedded Mouse liver tissue using Carboxylesterase 1/CES1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15388_rabbit-carboxylesterase-1-ces1-mab-7c9r6-52202517202984.jpg)
![Immunocytochemistry/ Immunofluorescence: Carboxylesterase 1/CES1 Antibody (7C9R6) [Carboxylesterase 1/CES1] - Confocal imaging of paraffin-embedded Rat liver tissue using Carboxylesterase 1/CES1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15388_rabbit-carboxylesterase-1-ces1-mab-7c9r6-52202517284992.jpg)
![Western Blot: CES1 Antibody [NBP1-00167] - Analysis of CES1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-00167 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CES1-Antibody-Western-Blot-NBP1-00167-img0005.jpg "Western Blot: CES1 Antibody [NBP1-00167] - Analysis of CES1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-00167 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: CES1 Antibody [NBP1-00167] - (2.5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-00167-img0004.jpg)
![Western Blot: Carboxylesterase 1/CES1 Antibody (SR1990) [NBP3-21940] - Western blot analysis using NBP3-21940 in U937 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21940_rabbit-carboxylesterase-1-ces1-mab-sr1990-672023106245.jpg "Western Blot: Carboxylesterase 1/CES1 Antibody (SR1990) [NBP3-21940] - Western blot analysis using NBP3-21940 in U937 cell lysate.")
![Western Blot: Carboxylesterase 1/CES1 Overexpression Lysate (Native) [NBL1-09112] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CES1.](http://images.novusbio.com/fullsize/CES1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09112-img0002.jpg "Western Blot: Carboxylesterase 1/CES1 Overexpression Lysate (Native) [NBL1-09112] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CES1.")
![Western Blot: Carboxylesterase 1/CES1 Overexpression Lysate (Native) [NBL1-09113] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CES1.](http://images.novusbio.com/fullsize/CES1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09113-img0002.jpg "Western Blot: Carboxylesterase 1/CES1 Overexpression Lysate (Native) [NBL1-09113] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CES1.")