ACAT2 Antibody (OTI3E2)

Images

 
Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] Analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2.
Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Human prostate tissue using anti-ACAT2 mouse monoclonal antibody.
Flow Cytometry: ACAT2 Antibody (3E2) [NBP2-02588] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACAT2 antibody, and then analyzed ...read more
Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACAT2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Human liver tissue using anti-ACAT2 mouse monoclonal antibody.
Flow Cytometry: ACAT2 Antibody (3E2) [NBP2-02588] - Analysis of Jurkat cells, using anti-ACAT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI3E2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ACAT2 Antibody (OTI3E2) Summary

Immunogen
Full length human recombinant protein of human ACAT2(NP_005882) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ACAT2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
41.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ACAT2 Knockout HeLa Cell Lysate

Reactivity Notes

Monkey

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for ACAT2 Antibody (OTI3E2)

  • acetoacetyl Coenzyme A thiolase
  • acetyl-CoA acetyltransferase 2
  • acetyl-CoA acetyltransferase, cytosolic
  • Acetyl-CoA transferase-like protein
  • acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase)
  • acetyl-Coenzyme A acetyltransferase 2
  • ACTL
  • Cytosolic acetoacetyl-CoA thiolase
  • EC 2.3.1
  • EC 2.3.1.9

Background

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ACAT2 Antibody (NBP2-02588) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70068AF350
Alexa Fluor 405 NBP2-70068AF405
Alexa Fluor 488 NBP2-70068AF488
Alexa Fluor 532 NBP2-70068AF532
Alexa Fluor 594 NBP2-70068AF594
Alexa Fluor 647 NBP2-70068AF647
Alexa Fluor 700 NBP2-70068AF700
Alexa Fluor 750 NBP2-70068AF750
Allophycocyanin NBP2-70068APC
Allophycocyanin/Cy7 NBP2-70068APCCY7
Biotin NBP2-70068B
DyLight 350 NBP2-70068UV
DyLight 405 NBP2-70068V
DyLight 488 NBP2-70068G
DyLight 550 NBP2-70068R
DyLight 594 NBP2-70068DL594
DyLight 650 NBP2-70068C
DyLight 680 NBP2-70068FR
DyLight 755 NBP2-70068IR
FITC NBP2-70068F
HRP NBP2-70068H
Janelia Fluor 549 NBP2-70068JF549
Janelia Fluor 646 NBP2-70068JF646
PE NBP2-70068PE
PE/Atto594 NBP2-70068PEATT594
PE/Cy5.5 NBP2-70068PECY55
PE/Cy7 NBP2-70068PECY7
PerCP NBP2-70068PCP

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Bioinformatics

Gene Symbol ACAT2
Entrez
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