Carboxylesterase 3/CES3/Esterase 31 Antibody

Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Carboxylesterase 3/CES3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carboxylesterase 3/CES3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Carboxylesterase 3/CES3/Esterase 31 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Carboxylesterase 3/CES3
  Tyr19-Glu561
  Accession # Q8VCT4
• Specificity: Detects recombinant mouse Carboxylesterase 3/CES3 in direct ELISAs and Western blots. Detects intrinsic CES3 in mouse and rat tissues in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CES1, rhCES2, recombinant mouse (rm) CES2, and rmCES5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CES3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxylesterase 3/CES3/Esterase 31 Antibody

• carboxylesterase 3 (brain)
• Carboxylesterase 3
• CES3
• EC 3.1.1
• EC 3.1.1.1
• ES31FLJ21736
• esterase 31
• Liver carboxylesterase 31 homolog

Background

Carboxylesterase 3 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). These carboxylesterases are widely distributed in mammalian tissues and have broad substrate specificity ranging from small molecule esters such as phenylester to long-chain fatty acid esters and thioesters. Because many ester-containing drugs require the ester linkage to improve the bioavailability of therapeutic agents, CESs play a major role in drug metabolism and activation. CES3 is also known as triacylglycerol hydrolase for its lipolytic activity. It has been reported to be the major lipase in white adipose tissue (3). Mouse CES3 shares 93% homology with rat CES3.

1. Redinbo, M. R. and Potter, P.M. (2005) Drug Discovery Today. 10:313.
2. Satoh, T. and Hosokawa, M. (2006) Chem.-Biol. Interactions. 162:195.
3. Soni, K. G. et al. (2004) J. Biol. Chem. 279:40683.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carboxylesterase 3/CES3/Esterase 31 Antibody (AF5985)(2)

Publications using AF5985

• MX Liu, M Gao, CZ Li, CZ Yu, H Yan, C Peng, Y Li, CG Li, ZL Ma, Y Zhao, MF Pu, LL Miao, XM Qi, J Ren Dicer1/miR-29/HMGCR axis contributes to hepatic free cholesterol accumulation in mouse non-alcoholic steatohepatitis Acta Pharmacol. Sin, 2017-01-23;0(0):. 1/23/2017 [PMID: 28112179]
• Yong Fan, Xin Fang, Asako Tajima, Xuehui Geng, Sarangarajan Ranganathan, Henry Dong et al. Evolution of Hepatic Steatosis to Fibrosis and Adenoma Formation in Liver-Specific Growth Hormone Receptor Knockout Mice Front Endocrinol (Lausanne) 2014-12-18 [PMID: 25566190]

Bioinformatics

• Gene Symbol: CES3
• Uniprot:
  • Q8VCT4()