LCAT Antibody

Western Blot: LCAT Antibody [NBP1-05950] - Analysis of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot analysis using rabbit antiserum against mouse LCAT.

Immunocytochemistry/Immunofluorescence: LCAT Antibody [NBP1-05950] - The LCAT antibody was tested at a 1:500 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

LCAT Antibody Summary

• Immunogen: Carboxyl-terminal histidine-tagged mouse recombinant LCAT purified from the medium of CHO cells overexpressing mouse LCAT cDNA. [UniProt# P16301]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LCAT
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
• Application Notes: This LCAT antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~62-65 kDa representing LCAT. Also has been shown to immunodeplete LCAT from mouse plasma, suggesting it should work fine for Immunoprecipitation (PMID: 18583707).
• Positive Control:

  LCAT Lysate (NBL1-12457)

Reactivity Notes

Mouse and human.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for LCAT Antibody

• EC 2.3.1.43
• lecithin-cholesterol acyltransferasePhospholipid-cholesterol acyltransferase
• phosphatidylcholine-sterol acyltransferase

Background

Lecithin: cholesterol acyltransferase (LCAT) is the plasma enzyme responsible for most HDL cholesteryl esters in human plasma. In species with cholesteryl ester transfer protein (CETP) it is also responsible for a significant proportion of VLDL and LDL cholesteryl esters. LCAT, through the esterification of cholesterol, plays a role in the reverse cholesterol transport pathway by facilitating the net movement of cholesterol from peripheral tissues back to the liver for excretion. LCAT deficiency results in low levels of plasma HDL, whereas a transgenic overexpression of LCAT results in increased plasma HDL concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LCAT Antibody (NBP1-05950)(3)

Publications using NBP1-05950

• Hirsch-Reinshagen, V et al. LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins. J Lipid Res;50(5):885-93. 2009 May. [PMID: 19065001]
• Mulya, A et al. Initial interaction of apoA-I with ABCA1 impacts in vivo metabolic fate of nascent HDL. J Lipid Res;49(11):2390-401. 2008 Nov. [PMID: 18583707]
• Lee, J et al. Functional LCAT deficiency in human apolipoprotein A-I transgenic, SR-BI knockout mice. J Lipid Res;48(5):1052-61. 2007 May. [PMID: 17272829]

Review for LCAT Antibody (NBP1-05950) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 11/12/2015

Summary

• Application: Western Blot
• Sample Tested: mouse liver
• Species: Mouse
• Lot: A1

Blocking

• Blocking Details: 5% milk TBST

Primary Anitbody

• Dilution Ratio: 1:1000 in 1% milk TBST 4 degree o/n

Secondary Antibody

• Secondary Description: IRDye 800CW Goat anti Rabbit IgG
• Secondary Manufacturer Cat#: 925-32211
• Secondary Concentration: 1:10000

Details

• Detection Notes: Licor

Bioinformatics

• Gene Symbol: LCAT
• Entrez:
  • 3931(Human)
  • 16816(Mouse)
• Uniprot:
  • P04180(Human)