LCAT Antibody

Images

 
Western Blot: LCAT Antibody [NBP1-05950] - Analysis of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on ...read more
Immunocytochemistry/ Immunofluorescence: LCAT Antibody [NBP1-05950] - The LCAT antibody was tested at a 1:500 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

LCAT Antibody Summary

Immunogen
Carboxyl-terminal histidine-tagged mouse recombinant LCAT purified from the medium of CHO cells overexpressing mouse LCAT cDNA. [UniProt# P16301]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LCAT
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This LCAT antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~62-65 kDa representing LCAT. Also has been shown to immunodeplete LCAT from mouse plasma, suggesting it should work fine for Immunoprecipitation (PMID: 18583707).
Positive Control
LCAT Lysate (NBL1-12457)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-05950 in the following applications:

Publications
Read Publications using NBP1-05950.

Reactivity Notes

Mouse and human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for LCAT Antibody

  • EC 2.3.1.43
  • lecithin-cholesterol acyltransferasePhospholipid-cholesterol acyltransferase
  • phosphatidylcholine-sterol acyltransferase

Background

Lecithin: cholesterol acyltransferase (LCAT) is the plasma enzyme responsible for most HDL cholesteryl esters in human plasma. In species with cholesteryl ester transfer protein (CETP) it is also responsible for a significant proportion of VLDL and LDL cholesteryl esters. LCAT, through the esterification of cholesterol, plays a role in the reverse cholesterol transport pathway by facilitating the net movement of cholesterol from peripheral tissues back to the liver for excretion. LCAT deficiency results in low levels of plasma HDL, whereas a transgenic overexpression of LCAT results in increased plasma HDL concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF

Publications for LCAT Antibody (NBP1-05950)(3)

Review for LCAT Antibody (NBP1-05950) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-05950:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot LCAT NBP1-05950
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reviewed by:
Anonymous
WB Mouse 11/12/2015
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Summary

ApplicationWestern Blot
Sample Testedmouse liver
SpeciesMouse
LotA1

Blocking

Blocking Details5% milk TBST

Primary Anitbody

Dilution Ratio1:1000 in 1% milk TBST 4 degree o/n

Secondary Antibody

Secondary DescriptionIRDye 800CW Goat anti Rabbit IgG
Secondary Manufacturer Cat#925-32211
Secondary Concentration1:10000

Details

Detection NotesLicor

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LCAT Antibody (NBP1-05950) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
11/12/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol LCAT
Entrez
Uniprot