Carbonic Anhydrase XII/CA12 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human kidney tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified ...read more
Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately 60 kDa (as indicated) using 10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Carbonic Anhydrase XII/CA12 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XII/CA12
Ala25-Gln291
Accession # O43570
Specificity
Detects human Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse Carbonic Anhydrase XII/CA12 is observed and less than 5% cross-reactivity with recombinant human CA1, 3, 5A, 5B, 6, 7, 8, and 10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CA12
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
Publications
Read Publication using
AF2190 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase XII/CA12 Antibody [Unconjugated]

  • CA12
  • Carbonate dehydratase XII
  • carbonic anhydrase 12
  • Carbonic Anhydrase XII
  • carbonic anhydrase XIICAXII
  • carbonic dehydratase
  • CA-XII
  • EC 4.2.1.1
  • FLJ20151
  • HsT18816
  • Tumor antigen HOM-RCC-3.1.3

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA12 is a type I membrane enzyme highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis (2, 3). It is over-expressed in some renal cell cancers and in glaucoma (2, 4). Two alternatively spliced forms exist, which either contains or lacks a 11 amino acid segment just before the transmembrane domain (5). The secreted, purified recombinant human CA12 contains the residues that are common for both forms.

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Tuereci, O. et al. (1998) Proc. Natl. Acad. Sci. USA 95:7608.
  3. Ivanov, S.V. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12596.
  4. Liao, S.-Y. et al. (2003) J. Med. Genet. 40:257.
  5. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carbonic Anhydrase XII/CA12 Antibody (AF2190)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Biotin BAF2190

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Bioinformatics

Gene Symbol CA12
Uniprot