Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA12.

Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free [NBP2-70329] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry-Paraffin - Carbonic Anhydrase XII/CA12 (OTI2C6)-Azide&BSA Free [NBP2-70329] - Carbonic Anhydrase XII/CA12 Immunofluorescence on Human Formalin-Fixed Paraffin-Embedded (FFPE) Breast tissue. FITC Conjugated version of antibody used (NBP2-70329F). Image from verified customer review.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI2C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free (NBP2-46042) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CA12(NP_001209) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CA12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin Validated for IHC-P from a verifed customer review.
  • Western Blot 1:500
• Theoretical MW: 36.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Carbonic Anhydrase XII/CA12 Antibody (OTI2C6) - Azide and BSA Free

• CA12
• Carbonate dehydratase XII
• carbonic anhydrase 12
• Carbonic Anhydrase XII
• carbonic anhydrase XIICAXII
• carbonic dehydratase
• CA-XII
• EC 4.2.1.1
• FLJ20151
• HsT18816
• Tumor antigen HOM-RCC-3.1.3

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CA12