Rat Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric)


Rat Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
31.25 - 2000 pg/mL
18.75 pg/mL

Order Details

Rat Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric) Summary

This kit recognizes Rat CA9 in samples. No significant cross-reactivity or interference between Rat CA9 and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
18.75 pg/mL
Assay Type
CV% < 5.41%
CV% < 5.83%
Spike Recovery
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage is content dependent.

Kit Components

  1. Substrate Reagent
  2. Stop Solution
  3. Micro ELISA Plate(Dismountable)
  4. HRP Conjugate Diluent
  5. Reference Standard & Sample Diluent
  6. Reference Standard
  7. Plate Sealer
  8. Product Description
  9. Concentrated Biotinylated Detection Ab (100×)
  10. Certificate of Analysis
  11. Concentrated HRP Conjugate (100×)
  12. Concentrated Wash Buffer (25×)
  13. Biotinylated Detection Ab Diluent

Alternate Names for Rat Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric)

  • CA9
  • CAIX
  • CA-IX
  • Carbonate dehydratase IX
  • carbonic anhydrase 9
  • Carbonic Anhydrase IX
  • carbonic dehydratase
  • EC
  • G250
  • Membrane antigen MN
  • MN
  • P54/58N
  • PMW1
  • RCC
  • RCC-associated antigen G250
  • RCC-associated protein G250
  • Renal cell carcinoma-associated antigen G250


Carbonic anhydrase alpha, isozyme IX, belongs to a family of zinc-containing metalloproteins which hydrate carbon dioxide to generate bicarbonate ions and protons (1). This main catalytic function allows carbonic anhydrase IX to participate in cellular pH regulation. The large family of carbonic anhydrase metalloproteins includes three major classes which have been identified based on sequence and structure analysis. The alpha class is a monomer found in mammals. The beta class may occur as a dimer, tetramer, hexamer or octamer and is found in plants, algae, and bacteria. Lastly, the gamma class is a trimer found in bacteria and represents the most ancient carbonic anhydrase. These three classes of carbonic anhydrase enzymes lack sequence or structural similarities, but all share a conserved active site zinc atom (1).

Carbonic anhydrase IX (theoretical molecular weight 50kDa) belongs to the monomeric alpha class and is a single pass-transmembrane protein with two extracellular domains which serve catalytic and cell adhesion functions (2, 3). By cooperating with sodium bicarbonate cotransporters (NBC), lactate and proton exporting monocarboxylic acid transporters (MCT), and a sodium/hydrogen exchanger (NHE), carbonic anhydrase IX is involved in pH regulation across the cell membrane. This functional property protects cancer cells from intracellular acidification and partly explains the role of carbonic anhydrase IX in cancer cell survival and proliferation. In contrast, the pH regulating activity of carbonic anhydrase IX induces extracellular acidification, which has been implicated in epithelial to mesenchymal transition (EMT) and promoting cancer invasion. Carbonic anhydrase IX is frequently overexpressed in cancer cells (e.g., colorectal-, breast-, lung-carcinoma and brain tumors), an effect promoted by hypoxia within the tumor microenvironment (4). An exception are tumors carrying pVHL inactivating mutations, such as clear cell renal cell carcinoma (ccRCC), where HIF-alpha is stabilized due to dysfunctional proteasomal targeting and can induce HRE (Hypoxia Response Element) containing genes even under physiological normoxia (5). Carbonic anhydrase IX may be detected by immunostaining in tumors, which is found in association with necrotic tissue and metastatic cells. Because the expression of carbonic anhydrase IX correlates with both tumor grade and stage, analysis of its expression in tumors serves as a prognostic factor (4, 6).


1. Tripp, B. C., Smith, K., & Ferry, J. G. (2001). Carbonic Anhydrase: New Insights for an Ancient Enzyme. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.R100045200

2. Nishimori, I., & Onishi, S. (2001). Carbonic anhydrase isozymes in the human pancreas. Digestive and Liver Disease. https://doi.org/10.1016/s1590-8658(01)80138-9

3. Zavadova, Z., & Zavada, J. (2005). Carbonic anhydrase IX (CA IX) mediates tumor cell interactions with microenvironment. Oncology Reports. https://doi.org/10.3892/or.13.5.977

4. Pastorekova, S., & Gillies, R. J. (2019). The role of carbonic anhydrase IX in cancer development: links to hypoxia, acidosis, and beyond. Cancer and Metastasis Reviews. https://doi.org/10.1007/s10555-019-09799-0

5. Haase, V. (2009). The VHL Tumor Suppressor: Master Regulator of HIF. Current Pharmaceutical Design. https://doi.org/10.2174/138161209789649394

6. Young, J. R., Coy, H., Kim, H. J., Douek, M., Sisk, A., Pantuck, A. J., & Raman, S. S. (2018). Association of the gross appearance of intratumoral vascularity at MDCT with the carbonic anhydrase IX score in clear cell renal cell carcinoma. American Journal of Roentgenology. https://doi.org/10.2214/AJR.18.19725


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, Bv, Ca, Fe, Ma, Pm, Pm, Rb, Sh, Xp
Applications: WB, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, TCS, KD, KO, LA
Species: Hu, Mu, Rt, Rb
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, Flow-IC
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
Species: Hu, Mu, Rt
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Mu
Applications: WB, Simple Western, IHC
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Species: Hu
Applications: Flow, IHC, IP, CyTOF-ready
Species: Hu
Species: All-NA
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Rt
Applications: ELISA

Publications for Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509) (0)

There are no publications for Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509) (0)

There are no reviews for Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Carbonic Anhydrase IX/CA9 Products

Bioinformatics Tool for Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509)

Discover related pathways, diseases and genes to Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509)

Discover more about diseases related to Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509).

Pathways for Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509)

View related products by pathway.

PTMs for Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509)

Learn more about PTMs related to Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509).

Research Areas for Carbonic Anhydrase IX/CA9 ELISA Kit (NBP2-72509)

Find related products by research area.

Blogs on Carbonic Anhydrase IX/CA9.

Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Rat Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric) and receive a gift card or discount.


Gene Symbol CA9