Rat Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric)

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Rat Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

Rat Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat CA9 in samples. No significant cross-reactivity or interference between Rat CA9 and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.41%
Intra-Assay
CV% < 5.83%
Spike Recovery
88-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CA9

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Substrate Reagent
  2. Stop Solution
  3. Micro ELISA Plate(Dismountable)
  4. HRP Conjugate Diluent
  5. Reference Standard & Sample Diluent
  6. Reference Standard
  7. Plate Sealer
  8. Product Description
  9. Concentrated Biotinylated Detection Ab (100×)
  10. Certificate of Analysis
  11. Concentrated HRP Conjugate (100×)
  12. Concentrated Wash Buffer (25×)
  13. Biotinylated Detection Ab Diluent

Alternate Names for Rat Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric)

  • CA9
  • CAIX
  • CA-IX
  • Carbonate dehydratase IX
  • carbonic anhydrase 9
  • Carbonic Anhydrase IX
  • carbonic dehydratase
  • EC 4.2.1.1
  • G250
  • Membrane antigen MN
  • MN
  • P54/58N
  • PMW1
  • RCC
  • RCC-associated antigen G250
  • RCC-associated protein G250
  • Renal cell carcinoma-associated antigen G250

Background

Carbonic anhydrase alpha, isozyme IX, belongs to a family of zinc-containing metalloproteins which hydrate carbon dioxide to generate bicarbonate ions and protons (1). This main catalytic function allows carbonic anhydrase IX to participate in cellular pH regulation. The large family of carbonic anhydrase metalloproteins includes three major classes which have been identified based on sequence and structure analysis. The alpha class is a monomer found in mammals. The beta class may occur as a dimer, tetramer, hexamer or octamer and is found in plants, algae, and bacteria. Lastly, the gamma class is a trimer found in bacteria and represents the most ancient carbonic anhydrase. These three classes of carbonic anhydrase enzymes lack sequence or structural similarities, but all share a conserved active site zinc atom (1).

Carbonic anhydrase IX (theoretical molecular weight 50kDa) belongs to the monomeric alpha class and is a single pass-transmembrane protein with two extracellular domains which serve catalytic and cell adhesion functions (2, 3). By cooperating with sodium bicarbonate cotransporters (NBC), lactate and proton exporting monocarboxylic acid transporters (MCT), and a sodium/hydrogen exchanger (NHE), carbonic anhydrase IX is involved in pH regulation across the cell membrane. This functional property protects cancer cells from intracellular acidification and partly explains the role of carbonic anhydrase IX in cancer cell survival and proliferation. In contrast, the pH regulating activity of carbonic anhydrase IX induces extracellular acidification, which has been implicated in epithelial to mesenchymal transition (EMT) and promoting cancer invasion. Carbonic anhydrase IX is frequently overexpressed in cancer cells (e.g., colorectal-, breast-, lung-carcinoma and brain tumors), an effect promoted by hypoxia within the tumor microenvironment (4). An exception are tumors carrying pVHL inactivating mutations, such as clear cell renal cell carcinoma (ccRCC), where HIF-alpha is stabilized due to dysfunctional proteasomal targeting and can induce HRE (Hypoxia Response Element) containing genes even under physiological normoxia (5). Carbonic anhydrase IX may be detected by immunostaining in tumors, which is found in association with necrotic tissue and metastatic cells. Because the expression of carbonic anhydrase IX correlates with both tumor grade and stage, analysis of its expression in tumors serves as a prognostic factor (4, 6).

References

1. Tripp, B. C., Smith, K., & Ferry, J. G. (2001). Carbonic Anhydrase: New Insights for an Ancient Enzyme. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.R100045200

2. Nishimori, I., & Onishi, S. (2001). Carbonic anhydrase isozymes in the human pancreas. Digestive and Liver Disease. https://doi.org/10.1016/s1590-8658(01)80138-9

3. Zavadova, Z., & Zavada, J. (2005). Carbonic anhydrase IX (CA IX) mediates tumor cell interactions with microenvironment. Oncology Reports. https://doi.org/10.3892/or.13.5.977

4. Pastorekova, S., & Gillies, R. J. (2019). The role of carbonic anhydrase IX in cancer development: links to hypoxia, acidosis, and beyond. Cancer and Metastasis Reviews. https://doi.org/10.1007/s10555-019-09799-0

5. Haase, V. (2009). The VHL Tumor Suppressor: Master Regulator of HIF. Current Pharmaceutical Design. https://doi.org/10.2174/138161209789649394

6. Young, J. R., Coy, H., Kim, H. J., Douek, M., Sisk, A., Pantuck, A. J., & Raman, S. S. (2018). Association of the gross appearance of intratumoral vascularity at MDCT with the carbonic anhydrase IX score in clear cell renal cell carcinoma. American Journal of Roentgenology. https://doi.org/10.2214/AJR.18.19725

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e...  Read full blog post.

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Bioinformatics

Gene Symbol CA9