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Carbonic Anhydrase IX/CA9 Antibody (303123) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IP, CyTOF-ready, ELISA(Cap)
Clone
303123
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2188U-100UG
Alexa Fluor 405
FAB2188V-100UG
Alexa Fluor 488
FAB2188G-100UG
Alexa Fluor 594
FAB2188T-100UG
Alexa Fluor 647
FAB2188R-100UG
Alexa Fluor 700
FAB2188N-100UG
Alexa Fluor 750
FAB2188S-100UG
Allophycocyanin
FAB2188A
Fluorescein
FAB2188F
Phycoerythrin
FAB2188P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB2188F
Carrier Free
FAB2188P
Carrier Free
FAB2188A
Carrier Free
MAB2188-SP
Carrier Free
MAB2188-500
Carrier Free
FAB2188V-100UG
Carrier Free
FAB2188U-100UG
Carrier Free
FAB2188T-100UG
Carrier Free
FAB2188S-100UG
Carrier Free
FAB2188R-100UG
Carrier Free
FAB2188N-100UG
Carrier Free
FAB2188G-100UG
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Carbonic Anhydrase IX/CA9 Antibody (303123) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX
Pro59-Asp414
Accession # Q16790
Specificity
Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CA9
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Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human Carbonic Anhydrase IX Biotinylated Antibody (Catalog number BAF2188)
Standard: Recombinant Human Carbonic Anhydrase IX/CA9 (Catalog number 2188-CA)
Publications
Read Publications using
MAB2188 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase IX/CA9 Antibody (303123) [Unconjugated]

  • CA9
  • CAIX
  • CA-IX
  • Carbonate dehydratase IX
  • carbonic anhydrase 9
  • Carbonic Anhydrase IX
  • carbonic dehydratase
  • EC 4.2.1.1
  • G250
  • Membrane antigen MN
  • MN
  • P54/58N
  • PMW1
  • RCC
  • RCC-associated antigen G250
  • RCC-associated protein G250
  • Renal cell carcinoma-associated antigen G250

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

  1. Pastorek, J. et al. (1994) Oncogene 9:2877.
  2. Opavsky, R. et al. (1996) Genomics 33:480.
  3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carbonic Anhydrase IX/CA9 Antibody (MAB2188)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, ICC.


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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB2188U
Alexa Fluor 405 FAB2188V
Alexa Fluor 488 FAB2188G
Alexa Fluor 594 FAB2188T
Alexa Fluor 647 FAB2188R
Alexa Fluor 700 FAB2188N
Alexa Fluor 750 FAB2188S
Allophycocyanin FAB2188A
Fluorescein FAB2188F
Phycoerythrin FAB2188P
Learn about Custom Antibody Labeling

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Blogs on Carbonic Anhydrase IX/CA9.

Check out the latest blog posts on Carbonic Anhydrase IX/CA9.
Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e...  Read full blog post.
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Bioinformatics

Gene Symbol CA9
Uniprot