Carbonic Anhydrase II/CA2 Overexpression Lysate

Western Blot: Carbonic Anhydrase II Overexpression Lysate (Adult Normal) [NBL1-08615] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Carbonic Anhydrase II.

• Reactivity: Hu
• Applications: WB

Carbonic Anhydrase II/CA2 Overexpression Lysate Summary

• Description:

  Carbonic Anhydrase II/CA2 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC201974
  
  Accession#: NM_000067
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: CA2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 29.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Carbonic Anhydrase II/CA2 Overexpression Lysate

• CA2
• CAII
• CA-IIEC 4.2.1.1
• Car2
• Carbonate dehydratase II
• carbonic anhydrase 2
• carbonic anhydrase B
• Carbonic anhydrase C
• Carbonic Anhydrase II
• carbonic anhydrase IICAC
• carbonic dehydratase

Background

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for Carbonic Anhydrase II/CA2 Lysate (NBL1-08615). (Showing 1 - 1 of 1 FAQ).

1. Do you know about how many mg or ug of CA II contained in this cell lysate?
   • Unfortunately, the exact amount of overexpressed protein has not been determined in any of our overexpression lysates. I am sorry for any inconvenience. The overexpression lysates are provided as 100ug of whole cell HEK 293 cell lysates transfected with either an overexpression or empty vector. Each lysate set will contain one positive control overexpression lysate, one negative control empty vector lysate, and one vial of 2 x sample buffer.

Bioinformatics

• Gene Symbol: CA2