Carbonic Anhydrase I/CA1 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin

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Carbonic Anhydrase I/CA1 Antibody [Biotin] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Carbonic Anhydrase I [Human Erythrocytes]. Uniprot ID P00915
Localization
Cytoplasmic
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ].
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CA1
Purity
Delipidation and Defibrination
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
Application Notes
This product has been assayed against 1.0 ug of Carbonic Anhydrase I in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:4,000 - 1:20,000 of the reconstitution concentration is suggested for this product.
Readout System

Reactivity Notes

Cross reactivity against Carbonic Anhydrase I from other tissues and species may occur but has not been specifically tested.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Alternate Names for Carbonic Anhydrase I/CA1 Antibody [Biotin]

  • CA1
  • CA-I
  • Car1
  • Carbonate dehydratase I
  • carbonic anhydrase 1
  • Carbonic anhydrase B
  • Carbonic Anhydrase I
  • carbonic anhydrase ICAB
  • carbonic dehydratase
  • EC 4.2.1.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CA1
Entrez