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Carbonic Anhydrase I/CA1 Antibody [HRP]

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Western Blot: Carbonic Anhydrase I/CA1 Antibody [HRP] [NB600-942] - Western Blot of Goat Carbonic Anhydrase I/CA1/CA1 antibody [HRP]. Lane 1: Opal Prestained Molecular Weight.Lane 2: Carbonic Anhydrase I/CA1 [10ug]. Primary antibody: Anti-Carbonic Anhydrase I/CA1 HRP Antibody at 1:1000 for 1 hr at RT.Secondary antibody: Streptavidin-HRP antibody at 1:40,000 for 30mins at RT.Blocking bufferPredicted/Observed size: ~29kDa.
Western Blot: Carbonic Anhydrase I/CA1 Antibody [HRP] [NB600-942] - Western Blot of Goat Carbonic Anhydrase I/CA1/CA1 antibody [HRP]. Lane 1: Opal Prestained Molecular Weight.Lane 2: Carbonic Anhydrase I/CA1 [10ug]. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
NBP1-77740-0.1mg
Unconjugated
NB120-6619-0.1mg
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Carbonic Anhydrase I/CA1 Antibody [HRP] Summary

Immunogen
Carbonic Anhydrase I/CA1/CA1 [Human Erythrocytes] (Uniprot: P00915)
Localization
Cytoplasmic
Specificity
Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CA1
Purity
Multi-step
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:20000
  • Immunohistochemistry 1:500-1:2500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000-1:5000
Application Notes
This product is tested by ELISA and western blot and useful in immunohistochemistry. Optimal titers should be obtained by researchers.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Readout System
Control
Carbonic Anhydrase I/CA1 Overexpression Lysate

Reactivity Notes

Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 100 ul deionized water (or equivalent)

Notes

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Carbonic Anhydrase I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ]

Alternate Names for Carbonic Anhydrase I/CA1 Antibody [HRP]

  • CA1
  • CA-I
  • Car1
  • Carbonate dehydratase I
  • carbonic anhydrase 1
  • Carbonic anhydrase B
  • Carbonic Anhydrase I
  • carbonic anhydrase ICAB
  • carbonic dehydratase
  • EC 4.2.1.1

Background

Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - analysis of Carbonic Anhydrase II protein (loading amount: 50 ng) using rabbit anti-Carbonic Anhydrase II antibody (NB600-919) at a dilution of 1:1000, incubation - overnight at 4C.
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Western Blot: GFAP Antibody [NB300-141] - Analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50 kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.
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Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB

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Control Lysate(s)

Carbonic Anhydrase I/CA1 Overexpression Lysate

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Bioinformatics

Gene Symbol CA1
Uniprot