Canine VEGF Quantikine ELISA Kit

Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Canine VEGF Quantikine ELISA Kit Summary

Background
The Quantikine Canine VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant canine VEGF and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine VEGF showed linear curves that were parallel to the standard curves obtained usi...ng the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine VEGF.
Show More
Specificity
Natural and recombinant canine VEGF. This assay cross-reacts with Human VEGF165.
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
VEGFA

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using CAVE00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Canine VEGF Quantikine ELISA Kit

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

Customers Who Viewed This Item Also Viewed...

233-FB/CF
Species: Hu
Applications: BA
AF321
Species: Hu
Applications: Block, CyTOF-ready, Flow, IHC, WB
AF644
Species: Mu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
NB100-105
Species: Bv, Ca, Fe, Ma, Hu, Pm, Mu, Po, Pm, Rb, Rt, Sh, Xp
Applications: ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, LA, PLA, Simple Western, TCS, WB
D6050
Species: Hu
Applications: ELISA
210-TA
Species: Hu
Applications: BA
923-AN/CF
Species: Hu
Applications: BA
AF3628
Species: Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, WB
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NB110-41083
Species: Hu
Applications: ChIP, ELISA, ICC/IF, WB
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
DPG00
Species: Hu
Applications: ELISA
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
294-HG/CF
Species: Hu
Applications: BA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for VEGF (CAVE00)(9)

We have publications tested in 4 confirmed species: Human, Bovine, Canine, Feline.


Filter By Application
All Applications
Filter By Species
Human
(1)
Bovine
(2)
Canine
(5)
Feline
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using CAVE00 Applications Species
HL Deissler, M Rehak, A Wolf Impairment of the Retinal Endothelial Cell Barrier Induced by Long-Term Treatment with VEGF-A165 No Longer Depends on the Growth Factor's Presence Biomolecules, 2022;12(5):. 2022 [PMID: 35625661] (Bovine) Bovine
F Sonderegge, A Nentwig, A Schweighau, T Francey, E Marti, J Mirkovitch, S Schuller Association of markers of endothelial activation and dysfunction with occurrence and outcome of pulmonary hemorrhage in dogs with leptospirosis Journal of veterinary internal medicine, 2021;0(0):. 2021 [PMID: 34076314] (Canine) Canine
T Zueva, R Morchón, E Carretón, JA Montoya-Al, A Santana, MD Bargues, S Mas-Coma, A Rodríguez-, F Simón Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms Parasit Vectors, 2019;12(1):315. 2019 [PMID: 31234915] (Canine) Canine
SH Lee, JC Ra, HJ Oh, MJ Kim, EMN Setyawan, YB Choi, JW Yang, SK Kang, SH Han, GA Kim, BC Lee Clinical Assessment of Intravenous Endothelial Progenitor Cell Transplantation in Dogs Cell Transplant, 2019;0(0):9636897188216. 2019 [PMID: 31018670] (Human) Human
LM Muellerlei, B Buxbaum, B Nell, DA Fux In-vitro binding analysis of anti-human vascular endothelial growth factor antibodies bevacizumab and aflibercept with canine, feline, and equine vascular endothelial growth factor Res. Vet. Sci., 2019;124(0):233-238. 2019 [PMID: 30933891] (Canine) Canine
HL Deissler, GK Lang, GE Lang Inhibition of Single Routes of Intracellular Signaling is Not Sufficient to Neutralize the Biphasic Disturbance of a Retinal Endothelial Cell Barrier Induced by VEGF-A165 Cell. Physiol. Biochem., 2017;42(4):1493-1513. 2017 [PMID: 28719888] (Bovine) Bovine
ES Bijsmans, RE Jepson, C Wheeler, HM Syme, J Elliott Plasma N-Terminal Probrain Natriuretic Peptide, Vascular Endothelial Growth Factor, and Cardiac Troponin I as Novel Biomarkers of Hypertensive Disease and Target Organ Damage in Cats J. Vet. Intern. Med., 2017;0(0):. 2017 [PMID: 28387019] (Feline) Feline
MW Nolan, MM Arkans, D LaVine, T DeFrancesc, JA Myers, EH Griffith, LP Posner, BW Keene, SP Tou, TL Gieger Pilot study to determine the feasibility of radiation therapy for dogs with right atrial masses and hemorrhagic pericardial effusion J Vet Cardiol, 2017;0(0):. 2017 [PMID: 28283317] (Canine) Canine
MW Nolan, TL Gieger, AA Karakashia, MN Nikolova-K, LP Posner, DM Roback, JN Rivera, S Chang Outcomes of Spatially Fractionated Radiotherapy (GRID) for Bulky Soft Tissue Sarcomas in a Large Animal Model Technol. Cancer Res. Treat, 2017;0(0):1533034617690. 2017 [PMID: 28168937] (Canine) Canine

Reviews for VEGF (CAVE00) (0)

There are no reviews for VEGF (CAVE00). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VEGF (CAVE00). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Additional VEGF Products

Bioinformatics Tool for VEGF (CAVE00)

Discover related pathways, diseases and genes to VEGF (CAVE00). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for VEGF (CAVE00)

Discover more about diseases related to VEGF (CAVE00).
 

Pathways for VEGF (CAVE00)

View related products by pathway.

PTMs for VEGF (CAVE00)

Learn more about PTMs related to VEGF (CAVE00).

Blogs on VEGF. Showing 1-10 of 22 blog posts - Show all blog posts.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit.  ...  Read full blog post.

Showing 1-10 of 22 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Canine VEGF Quantikine ELISA Kit and receive a gift card or discount.

Bioinformatics

Gene Symbol VEGFA