Canine VEGF Quantikine ELISA Kit

• Reactivity: Ca
• Applications: ELISA
• Conjugate: HRP

Canine VEGF Quantikine ELISA Kit Summary

• Background: The Quantikine Canine VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant canine VEGF and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine VEGF showed linear curves that were parallel to the standard curves obtained usi...ng the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine VEGF.
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• Specificity: Natural and recombinant canine VEGF. This assay cross-reacts with Human VEGF₁₆₅.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: VEGFA

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Canine VEGF Quantikine ELISA Kit

• MVCD1
• VAS
• vascular endothelial growth factor A
• Vascular permeability factor
• Vasculotropin
• VEGF
• VEGFA
• VEGF-A
• VEGFMGC70609
• VPF
• VPFvascular endothelial growth factor

Background

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for VEGF (CAVE00)(9)

Publications using CAVE00

• HL Deissler, M Rehak, A Wolf Impairment of the Retinal Endothelial Cell Barrier Induced by Long-Term Treatment with VEGF-A165 No Longer Depends on the Growth Factor's Presence Biomolecules, 2022-05-23;12(5):. 2022-05-23 [PMID: 35625661] (Bovine)
• F Sonderegge, A Nentwig, A Schweighau, T Francey, E Marti, J Mirkovitch, S Schuller Association of markers of endothelial activation and dysfunction with occurrence and outcome of pulmonary hemorrhage in dogs with leptospirosis Journal of veterinary internal medicine, 2021-06-02;0(0):. 2021-06-02 [PMID: 34076314] (Canine)
• T Zueva, R Morchón, E Carretón, JA Montoya-Al, A Santana, MD Bargues, S Mas-Coma, A Rodríguez-, F Simón Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms Parasit Vectors, 2019-06-24;12(1):315. 2019-06-24 [PMID: 31234915] (Canine)
• SH Lee, JC Ra, HJ Oh, MJ Kim, EMN Setyawan, YB Choi, JW Yang, SK Kang, SH Han, GA Kim, BC Lee Clinical Assessment of Intravenous Endothelial Progenitor Cell Transplantation in Dogs Cell Transplant, 2019-04-24;0(0):9636897188216. 2019-04-24 [PMID: 31018670] (Human)
• LM Muellerlei, B Buxbaum, B Nell, DA Fux In-vitro binding analysis of anti-human vascular endothelial growth factor antibodies bevacizumab and aflibercept with canine, feline, and equine vascular endothelial growth factor Res. Vet. Sci., 2019-03-22;124(0):233-238. 2019-03-22 [PMID: 30933891] (Canine)
• HL Deissler, GK Lang, GE Lang Inhibition of Single Routes of Intracellular Signaling is Not Sufficient to Neutralize the Biphasic Disturbance of a Retinal Endothelial Cell Barrier Induced by VEGF-A165 Cell. Physiol. Biochem., 2017-07-18;42(4):1493-1513. 2017-07-18 [PMID: 28719888] (Bovine)
• ES Bijsmans, RE Jepson, C Wheeler, HM Syme, J Elliott Plasma N-Terminal Probrain Natriuretic Peptide, Vascular Endothelial Growth Factor, and Cardiac Troponin I as Novel Biomarkers of Hypertensive Disease and Target Organ Damage in Cats J. Vet. Intern. Med., 2017-04-07;0(0):. 2017-04-07 [PMID: 28387019] (Feline)
• MW Nolan, MM Arkans, D LaVine, T DeFrancesc, JA Myers, EH Griffith, LP Posner, BW Keene, SP Tou, TL Gieger Pilot study to determine the feasibility of radiation therapy for dogs with right atrial masses and hemorrhagic pericardial effusion J Vet Cardiol, 2017-03-07;0(0):. 2017-03-07 [PMID: 28283317] (Canine)
• MW Nolan, TL Gieger, AA Karakashia, MN Nikolova-K, LP Posner, DM Roback, JN Rivera, S Chang Outcomes of Spatially Fractionated Radiotherapy (GRID) for Bulky Soft Tissue Sarcomas in a Large Animal Model Technol. Cancer Res. Treat, 2017-02-07;0(0):1533034617690. 2017-02-07 [PMID: 28168937] (Canine)

FAQs for VEGF (CAVE00). (Showing 1 - 1 of 1 FAQs).

1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
   • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Bioinformatics

• Gene Symbol: VEGFA