Calreticulin Antibody (1G6A7)

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Western Blot: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of Alexa Fluor (R) 488 conjugate of NBP1-47518. Analysis using Calreticulin mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control ...read more
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of Alexa Fluor (R) 488 conjugate of NBP1-47518. The Calreticulin antibody was tested in HeLa cells at a 1:250 dilution ...read more
Immunohistochemistry-Paraffin: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of Alexa Fluor (R) 488 conjugate of NBP1-47518. Immunohistochemical paraffin-embedded human placenta tissues using anti-Calreticulin ...read more
Flow Cytometry: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of Alexa Fluor (R) 488 conjugate of NBP1-47518. Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Calreticulin ...read more
Western Blot: Calreticulin Antibody (1G6A7) [NBP1-47518] - Western blot analysis of whole cell lysates from cell lines namely Hela, A549, NTERA2 and MCF-7 using Calreticulin antibody clone 1G6A7. The antibody generated ...read more
Western Blot: Calreticulin Antibody (1G6A7) [NBP1-47518] - Western blot analysis using Calreticulin mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Calreticulin cDNA (2).
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) [NBP1-47518] - Confocal immunofluorescence analysis of 3T3-L1 cells using anti-Calreticulin mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) [NBP1-47518] - Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using anti-Calreticulin mAb (green). Red: Actin ...read more
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) [NBP1-47518] - The Calreticulin antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Actin was counterstained against ...read more
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of Alexa Fluor (R) 488 conjugate of NBP1-47518. Confocal immunofluorescence 3T3-L1 cells using anti-Calreticulin mAb ...read more
Immunohistochemistry-Paraffin: Calreticulin Antibody (1G6A7) [NBP1-47518] - Immunohistochemical analysis of paraffin-embedded human placenta tissues using anti-Calreticulin mAb.
Flow Cytometry: Calreticulin Antibody (1G6A7) [NBP1-47518] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Calreticulin antibody (dark blue). Isotype control shown in orange. An ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
1G6A7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Calreticulin Antibody (1G6A7) Summary

Immunogen
A synthetic peptide corresponding to the C-terminus (EEEDVPGQAKDELC) of human Calreticulin, conjugated to KLH. [UniProt# P27797]
Marker
Endoplasmic Reticulum Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CALR
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This Calreticulin (1G6A7) antibody is useful in Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
MCF-7 Lysate (NB820-59465)
NTERA-2 Lysate (NBP1-44968)

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Calreticulin Antibody (1G6A7)

  • Autoantigen Ro
  • CALR
  • Calregulin
  • Calreticulin
  • cC1qR
  • CRP55
  • CRT
  • CRTC
  • Endoplasmic reticulum resident protein 60
  • ERp60
  • FLJ26680
  • grp60
  • RO
  • Sicca syndrome antigen A (autoantigen Ro; calreticulin)
  • SSA
  • SSAHACBP
  • Vasostatin

Background

Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however, it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Calreticulin.

Calreticulin - ER chaperone involved in calcium homeostasis and protein quality control
Calreticulin is a calcium-dependent ER chaperone, involved in protein folding, maturation, and cellular localization. Calreticulin is a highly conserved 48 kDa protein encoded by the CALR gene. Calreticulin and its homolog calnexin regulate the fo...  Read full blog post.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

Calreticulin: a Multiprocess Calcium Buffering Chaperone
Calreticulin is a Calcium binding chaperone that has multiple functions both inside and outside the endoplasmic reticulum. Calreticulin is involved in the quality control of newly synthesized proteins and glycoproteins, interacting with various other...  Read full blog post.

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Bioinformatics

Gene Symbol CALR
Entrez
Uniprot