C-Peptide Antibody (915612) [Unconjugated]

C-Peptide was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human C-Peptide Monoclonal Antibody (Catalog # MAB80561) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 915612
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

C-Peptide Antibody (915612) [Unconjugated] Summary

• Immunogen: Human C-Peptide synthetic peptide
  EAEDLQVGQVELGGGPGAGSLQPLALEGSLQ
  Accession # P01308
• Specificity: Detects human C-Peptide in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: INS
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for C-Peptide Antibody (915612) [Unconjugated]

• C-P
• C-Peptide
• IDDM2
• ILPR
• Insulin C-Peptide
• insulin
• IRDN
• MODY10
• Proinsulin C-Peptide
• proinsulin

Background

Insulin is a peptide hormone that facilitates the cellular uptake of glucose by regulating the appearance of membrane glucose transporters. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 25-54), a C-peptide (aa 55-89), and a 21 aa A chain (aa 90-110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfide-linked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes. The human Insulin C-peptide shares 61% and 68% aa sequence identity with mouse and rat Insulin C-peptide, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for C-Peptide Antibody (MAB80561)(1)

Publication using MAB80561

• Aleman, J;Ravikumar, K;Wiegand, C;Schurdak, ME;Vernetti, L;Gavlock, D;Reese, C;DeBiasio, R;LaRocca, G;Angarita, YD;Gough, A;Soto-Gutierrez, A;Behari, J;Yechoor, V;Miedel, MT;Stern, AM;Banerjee, I;Taylor, DL; A metabolic-dysfunction associated steatotic liver acinus biomimetic induces pancreatic islet dysfunction in a coupled microphysiology system bioRxiv : the preprint server for biology 2024-04-30 [PMID: 38712135] (Immunohistochemistry, Human)

Bioinformatics

• Gene Symbol: INS
• Uniprot:
  • P01308()