c-Myb [p Thr486] Antibody

Images

 
Western Blot: c-Myb [p Thr486] Antibody [NBP2-21659] - Rabbit c-Myb pT486 antibody. Lane 1: Cos7 cells were either null for c-Myb. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

c-Myb [p Thr486] Antibody Summary

Immunogen
Synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486. Protein accession number 110556654.
Modification
p Thr486
Specificity
Cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein. This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500
  • ELISA 1:5000-1:16000
Application Notes
This antibody has been tested for use in ELISA and Western Blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb. Specific conditions for reactivity and detection of phosphorylated c-Myb should be optimized by the end user. Expect a band approximately ~75 kDa in size corresponding to phosphorylated c-Myb by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Reactive in Mouse. Expect reactivity with c-Myb from Rat, Porcine, Human, Equine, Platypus, Opossum, Orangutan, Spider Monkey, Bonobo, Gorilla, and Chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from Human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (Avian), both from Cattle, all based on a 93% identity with the immunizing sequence.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for c-Myb [p Thr486] Antibody

  • c-myb protein (140 AA)
  • Cmyb
  • c-myb
  • c-myb_CDS
  • c-myb10A_CDS
  • c-myb13A_CDS
  • c-myb14A_CDS
  • c-myb8B_CDS
  • efg
  • Proto-oncogene c-Myb
  • transcriptional activator Myb
  • v-myb avian myeloblastosis viral oncogene homolog
  • v-myb myeloblastosis viral oncogene homolog (avian)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NBP2-33930
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP1-90171
Species: Hu
Applications: IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-381
Species: Hu
Applications: WB, ChIP, IP, PLA
MAB17791
Species: Hu, Mu
Applications: WB
NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
AF5414
Species: Hu
Applications: WB, Simple Western
NBP2-27163
Species: Hu, Mu, Bv, Ca, Eq, Pm
Applications: WB, Flow
NBP1-43461
Species: Mu
Applications: Flow, IHC, IP
MAB6799
Species: Hu
Applications: WB, IHC
NBP2-01223
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB600-233
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, PLA
NBP2-45745
Species: Hu
Applications: WB, IHC, IF
AF1356
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready

Publications for c-Myb Antibody (NBP2-21659) (0)

There are no publications for c-Myb Antibody (NBP2-21659).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for c-Myb Antibody (NBP2-21659) (0)

There are no reviews for c-Myb Antibody (NBP2-21659). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for c-Myb Antibody (NBP2-21659) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional c-Myb Products

Bioinformatics Tool for c-Myb Antibody (NBP2-21659)

Discover related pathways, diseases and genes to c-Myb Antibody (NBP2-21659). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for c-Myb Antibody (NBP2-21659)

Discover more about diseases related to c-Myb Antibody (NBP2-21659).
 

Pathways for c-Myb Antibody (NBP2-21659)

View related products by pathway.

PTMs for c-Myb Antibody (NBP2-21659)

Learn more about PTMs related to c-Myb Antibody (NBP2-21659).
 

Research Areas for c-Myb Antibody (NBP2-21659)

Find related products by research area.

Blogs on c-Myb

There are no specific blogs for c-Myb, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our c-Myb [p Thr486] Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol MYB
Entrez
Uniprot