c-Abl Antibody (M209) - BSA Free

Western Blot: c-Abl Antibody (M209) [NBP3-23312] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).

Western Blot: c-Abl Antibody (M209) [NBP3-23312] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: M209
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

c-Abl Antibody (M209) - BSA Free Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Clone (M209) was generated from a recombinant protein corresponding to the C-terminal region of human c-Abl. [UniProt# P00519]
• Specificity: This antibody detects a 145 kDa protein corresponding to c-Abl on SDS-PAGE immunoblots of human K-562 and HL-60 cells, and mouse ANN-1 cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABL1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 145 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.2% gelatin
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for c-Abl Antibody (M209) - BSA Free

• Abelson murine leukemia viral oncogene homolog 1
• ABL
• ABL1
• bcr/abl
• BCR-ABL1
• c-abl oncogene 1, non-receptor tyrosine kinase
• c-abl oncogene 1, receptor tyrosine kinase
• cAbl
• c-Abl
• EC 2.7.10
• EC 2.7.10.2
• JTK7
• JTK7bcr/abl
• p150bcr/c-abl oncogene protein
• Proto-oncogene c-Abl
• proto-oncogene tyrosine-protein kinase ABL1
• tyrosine-protein kinase ABL1
• v-abl Abelson murine leukemia viral oncogene homolog 1
• v-abl

Background

c-Abl is the human cellular homolog of a the v-Abl viral oncogene. v-Abl was originally discovered as a mouse cell-derived sequence in the genome of the Abelson murine leukemia virus (A-MuLV), a transforming retrovirus isolated by the laboratory of Dr. H.T. Abelson. c-Abl is a tyrosine kinase from the Src-family of tyrosine kinases that localizes to both the cytoplasm and nucleus. It bears an SH2 (src-homology 2) and SH3 (src-homology 3) domain responsible for mediating c-Abl protein-protein interactions. c-Abl is implicated to play a role in multiple cellular processes such as cell cycle checkpoint signaling, apoptosis, cell differentiation, cell adhesion, and transcriptional regulation. Chromosomal translocations involving the c-Abl gene are associated with human leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABL1