BubR1 Antibody (S05-3G7)

Western Blot: BubR1 Antibody (S05-3G7) [NBP3-19840] - Western blot detection of BubR1 in CHO-K1, Ramos cell lysates using NBP3-19840 (1:1000 diluted). Predicted band size: 120kDa. Observed band size: 120kDa.

Immunohistochemistry-Paraffin: BubR1 Antibody (S05-3G7) [NBP3-19840] - Immunohistochemistry of BubR1 in paraffin-embedded Human lung cancer tissue using NBP3-19840 at dilution 1/50

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S05-3G7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

BubR1 Antibody (S05-3G7) Summary

• Description: Novus Biologicals Rabbit BubR1 Antibody (S05-3G7) (NBP3-19840) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human BubR1 (Uniprot # O60566)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BUB1B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000-1/10000
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for BubR1 Antibody (S05-3G7)

• Bub1A
• BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
• budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• EC 2.7.11.1
• hBUBR1
• MAD3/BUB1-related protein kinase
• MAD3LBUB1beta
• Mitotic checkpoint kinase MAD3L
• Protein SSK1
• SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), also known as BubR1, is a kinase involved in spindle checkpoint function. BUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. BubR-1 localizes to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation.

BubR1 antibodies are useful tools for cancer research and cell division studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BUB1B