Brk [p Tyr342] Antibody - BSA Free

Western Blot: Brk [p Tyr342] Antibody [NBP2-29687] - IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk(y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(y342).

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Brk [p Tyr342] Antibody - BSA Free Summary

• Description: Relevant Entrez Gene Numbers: NM_005975.2
• Immunogen: KLH conjugated synthetic peptide corresponding to amino acids encompassing Tyr342 of human Brk.
• Modification: p Tyr342
• Specificity: Recognizes human phospho-Brk at tyrosine 342.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTK6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M Tris-Glycine (pH 7.4) and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Relevant Entrez Gene Numbers: NM_005975.2

Alternate Names for Brk [p Tyr342] Antibody - BSA Free

• Breast tumor kinase
• Brk
• BRKprotein-tyrosine kinase 6
• EC 2.7.10
• EC 2.7.10.2
• FLJ42088
• protein-tyrosine kinase BRK
• PTK6 protein tyrosine kinase 6
• PTK6
• Sik
• Tksk
• Tyrosine-protein kinase BRK

Background

Brk is encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTK6
• Uniprot:
  • Q13882()