STAP2 Antibody

Western Blot: STAP2 Antibody [NB300-987] - NB300-987 (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: STAP2 Antibody [NB300-987] - (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: STAP2 Antibody [NB300-987] - (0.03ug/ml) staining of human heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

STAP2 Antibody Summary

• Immunogen: Peptide with sequence C-ELQKKLEKRRALEH corresponding to C-Terminus according to NP_060190.2, NP_001013863.1.
• Specificity: This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STAP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.05 - 0.3 ug/ml
• Application Notes: WB: Approx. 48 kDa band observed in human heart lysates (calculated MW of 49.6 kDa band according to NP_060190.2). IHC-P: Human brain (cortex) shows textured cytoplasm staining in neuronal bodies and processes.
• Control:

  STAP2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for STAP2 Antibody

• BKSbrk kinase substrate
• Breast tumor kinase substrate
• BRK substrate
• FLJ20234
• signal transducing adaptor family member 2
• signal-transducing adaptor protein 2
• signal-transducing adaptor protein-2
• STAP-2

Background

This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAP2 Antibody (NB300-987)(5)

Publications using NB300-987

• Ikeda O, Mizushima A, Sekine Y, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T. Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells. Cancer Sci;102(4):756-61. 2011-04-01 [PMID: 21205088]
• Ikeda O, Sekine Y, Mizushima A, Nakasuji M, Miyasaka Y, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T. J Biol Chem;285(49):38093-103. 2010-12-03 [PMID: 20929863]
• Sekine Y, Ikeda O, Tsuji S et al. Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells J Immunol 2009-12-15 [PMID: 19933863]
• Ikeda O, Miyasaka Y, Sekine Y, Mizushima A, Muromoto R, Nanbo A, Yoshimura A, Matsuda T. STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation. Biochem Biophys Res Commun;384(1):71-5. 2009-06-19 [PMID: 19393627]
• Minoguchi M, Minoguchi S, Aki D et al. STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation inacute-phase response through its YXXQ motif. J Biol Chem 2003-03-28 [PMID: 12540842]

Bioinformatics

• Gene Symbol: STAP2
• Entrez:
  • 55620(Human)
• Uniprot:
  • Q9UGK3(Human)