CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Brk was detected in immersion fixed paraffin-embedded sections of human lymphoma using 15 µg/mL Goat Anti-Human Brk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3966) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3966-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89936-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29687-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89935-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98347-50ul | |||
![Immunohistochemistry-Paraffin: Brk Antibody (2F11) [H00005753-M01] - Analysis of monoclonal antibody to PTK6 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Brk-Antibody-2F11-Immunohistochemistry-Paraffin-H00005753-M01-img0003.jpg "Immunohistochemistry-Paraffin: Brk Antibody (2F11) [H00005753-M01] - Analysis of monoclonal antibody to PTK6 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.")
![Immunohistochemistry: Brk Antibody [NBP1-89936] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells and decidua cells.](http://images.novusbio.com/fullsize/Brk-Antibody-Immunohistochemistry-NBP1-89936-img0003.jpg "Immunohistochemistry: Brk Antibody [NBP1-89936] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells and decidua cells.")
![Western Blot: Brk Antibody (2H12B8) [NB110-60537] - Analysis using PTK6 mouse mAb against Hela (1), A549 (2) and MCF-7 (3) cell lysate.](http://images.novusbio.com/fullsize/Brk-Antibody-2H12B8-Western-Blot-NB110-60537-img0004.jpg "Western Blot: Brk Antibody (2H12B8) [NB110-60537] - Analysis using PTK6 mouse mAb against Hela (1), A549 (2) and MCF-7 (3) cell lysate.")
![Western Blot: Mouse Monoclonal Brk Antibody (2H12B8) [NB110-60537] - Whole cell lysates from MDA-MB-231 cells that were exposed to 20% or 1% O2 for 48 hours were loaded with 50 ug/lane. 10% SDS-PAGE. Brk Antibody (NB110-60537) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb110-60537_mouse-monoclonal-brk-antibody-2h12b8-western-blot-2122024112532.jpg)
![Western Blot: Brk Overexpression Lysate (Adult Normal) [NBL1-14954] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Brk.](http://images.novusbio.com/fullsize/Brk-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14954-img0002.jpg "Western Blot: Brk Overexpression Lysate (Adult Normal) [NBL1-14954] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Brk.")
![Western Blot: Brk [p Tyr342] Antibody [NBP2-29687] - IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk(y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(y342).](http://images.novusbio.com/fullsize/Brk-[p-Tyr342]-Antibody-Western-Blot-NBP2-29687-img0002.jpg "Western Blot: Brk [p Tyr342] Antibody [NBP2-29687] - IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk(y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(y342).")
