ERK5/BMK1 Antibody [Unconjugated]

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Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
ERK5/BMK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2848) at 15 µg/mL for 3 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

ERK5/BMK1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ERK5/BMK1
Met1-Glu432
Accession # Q13164
Specificity
Detects human and mouse ERK5/BMK1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAPK7
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry 10-25 ug/mL
Publications
Read Publications using
AF2848 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ERK5/BMK1 Antibody [Unconjugated]

  • Big MAP kinase 1
  • BMK1 kinase
  • BMK1
  • BMK-1
  • BMK1EC 2.7.11.24
  • EC 2.7.11
  • ERK5
  • ERK-5
  • ERK5MAPK 7
  • Extracellular signal-regulated kinase 5
  • extracellular-signal-regulated kinase 5
  • MAP kinase 7
  • MAPK7
  • mitogen-activated protein kinase 7
  • PRKM7ERK4

Background

Extracellular signal-regulated kinase 5 (ERK5), also known as big mitogen-activated protein kinase 1 (BMK1) and MAPK7, is activated by a several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation. ERK5’s upstream MAPK kinase has been identified as MEK5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ERK5/BMK1 Antibody (AF2848)(3)

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Bioinformatics

Gene Symbol MAPK7
Uniprot