BRD8 Antibody - BSA Free

Western Blot: BRD8 Antibody [NB100-79780] - Detection of Human BRD8 by Western Blot. Samples: Nuclear extract (50 and 15 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-BRD8 antibody NB100-79780 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: BRD8 Antibody [NB100-79780] - Detection of human BRD8 by western blot of immunoprecipitates. Samples: Nuclear extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody NB100-79780 used for IP at 6 ug per reaction. BRD8 was also immunoprecipitated by rabbit anti-BRD8 antibody NB100-79781. For blotting immunoprecipitated BRD8, NB100-79780 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

BRD8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BRD8 Antibody - BSA Free (NB100-79780) is a polyclonal antibody validated for use in WB and IP. Anti-BRD8 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 25 and 75 of human Bromodomain containing 8 using the numbering given in TrEMBL entry Q969M9 (GeneID 10902).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BRD8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BRD8 Antibody - BSA Free

• BRD8
• bromodomain containing 8
• bromodomain-containing protein 8
• Skeletal muscle abundant protein
• SMAP
• SMAP2
• SMAPp120Skeletal muscle abundant protein 2
• Thyroid hormone receptor coactivating protein of 120 kDa
• TrCP120

Background

The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Three alternatively spliced transcript variants that encode distinct isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BRD8 Antibody (NB100-79780)(2)

Publications using NB100-79780

• Gevry N, Hardy S, Jacques PE et al. Histone H2A.Z is essential for estrogen receptor signaling. Genes Dev 2009-07-01 [PMID: 19515975]
• van Beekum O, Brenkman AB, Grontved L et al. The adipogenic acetyltransferase Tip60 targets activation function 1 of peroxisome proliferator-activated receptor gamma. Endocrinology 2008-04-01 [PMID: 18096664]

Bioinformatics

• Gene Symbol: BRD8
• Entrez:
  • 10902(Human)
• Uniprot:
  • Q969M9(Human)