BRD4 Antibody (CL1118)

Images

 
Western Blot: BRD4 Antibody (CL1118) [NBP2-52959] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunohistochemistry-Paraffin: BRD4 Antibody (CL1118) [NBP2-52959] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules and in glomeruli.
Immunohistochemistry-Paraffin: BRD4 Antibody (CL1118) [NBP2-52959] - Staining of human stomach cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: BRD4 Antibody (CL1118) [NBP2-52959] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: BRD4 Antibody (CL1118) [NBP2-52959] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
CL1118
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

BRD4 Antibody (CL1118) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPP
Epitope
EAPSPLMIHSPQMSQ
Specificity
Specificity of human BRD4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BRD4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control
BRD4 Knockout 293T Cell Lysate
Control Peptide

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for BRD4 Antibody (CL1118)

  • bromodomain containing 4
  • bromodomain-containing 4
  • CAPchromosome-associated protein
  • HUNK1bromodomain-containing protein 4
  • HUNKI
  • MCAP
  • Protein HUNK1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BRD4 Antibody (NBP2-52959) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati...  Read full blog post.

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Bioinformatics

Gene Symbol BRD4