BP1 Antibody


Western Blot: BP1 Antibody [NB100-481] - Detection of MDA-MB-231 cell lysates.
Immunohistochemistry: BP1 Antibody [NB100-481] - Top Panel: BP1 antibody detected in breast cancer cells. Bottom Panel: Staining of normal breast cancer cells.
Immunoprecipitation: BP1 Antibody [NB100-481] - Antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 ug of cell extract using anti-BP1 (NB100-481) antibody diluted 1:1,000 3. Negative ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
1 mg/ml

BP1 Antibody Summary

A synthetic peptide made specifically to the human BP1 protein sequence (between residues 1-60) [UniProt# Q92988].
This is specific for human BP1 (DLX4 variant 1). Berg PE, Kirolikar S. DLX4 (distal-less homeobox 4). Atlas Genet Cytogenet Oncol Haematol. January 2011. URL : http://AtlasGeneticsOncology.org/Genes/DLX4ID49827ch17q21.html
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This BP1 antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western blot, where a band is seen at ~36 kDa. IP has been done with 35S-labeled BP1 protein only. It is not known if this antibody will immunoprecipitate a cell extract. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
NB100-481 in the following applications:

Read Publications using
NB100-481 in the following applications:

  • WB
    4 publications

Reactivity Notes

Immunogen sequence has 82% homology with mouse.

Alternate Names for BP1 Antibody

  • Beta protein 1
  • BP1distal-less homeo box 7
  • distal-less homeobox 4
  • DLX7distal-less homeo box 9
  • DLX8DLX9
  • homeobox protein DLX-4
  • Homeobox protein DLX-7
  • Homeobox protein DLX-8


BP1 (beta protein 1; also called distal-less homeobox gene 4 or DLX4) is a member of DLX family of homeobox genes and it controls many aspects of embryonic development including bone morphogenesis, skeletal patterning and cell differentiation. Originally discovered to be a repressor of beta-globin gene in erythroid cells, BP1 is normally expressed during early hematopoiesis with complete absence from most normal adult tissues and is widely re-expressed in leukemias, lung, breast, ovarian as well as prostate cancers. BP1 is an oncogene with a major role in breast cancer, and that its expression correlates with breast cancer progression and invasion. BP1 has been found to be detectable in 80% of infiltrating ductal carcinomas, but undetectable in matched normal controls, being the first DLX gene to be strongly implicated in breast cancer. Furthermore, expression of DLX4 correlates with estrogen receptor status and also cancer progression and invasion in breast cancer.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BP1 Antibody (NB100-481)(5)

Reviews for BP1 Antibody (NB100-481) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-481:
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Images Ratings Applications Species Date Details
reviewed by:
WB Human 08/10/2010


ApplicationWestern Blot
Sample Testedwhole cell lysate, Sample Amount: 10-20ug/well


Blocking DetailsBlocking Buffer: 5% in TNET, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:5000, Incubation Dilution Buffer: TNET, Incubation Time: overnight, Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-Rabbit, Secondary Ab Dilution Ratio: 1:15000
Secondary Manufacturer Cat#Pierce #1858415


Detection NotesDetection Method: Chemiluminescence, Exposure Time: 5-30 min, Specific Bands: 37 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BP1 Antibody (NB100-481) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-481AF405
Alexa Fluor (R) 488 Labeled NB100-481AF488
Alexa Fluor (R) 647 Labeled NB100-481AF647
Alexa Fluor (R) 700 Labeled NB100-481AF700
Biotin Labeled NB100-481B
DyLight 405 Labeled NB100-481V
DyLight 488 Labeled NB100-481G
DyLight 550 Labeled NB100-481R
DyLight 650 Labeled NB100-481C
FITC Labeled NB100-481F
HRP Labeled NB100-481H

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Blogs on BP1.

BRCA1 - A Critical Tumor Suppressor Gene in Women
Breast cancer 1, early onset (BRCA1) is a well-known tumor suppressor gene that was originally discovered due to its link with early-onset breast and ovarian cancer in women. The BRCA1 protein contains the following domains: RING finger, RAD51-interac...  Read full blog post.

BP1 Antibodies, Beta Globin and Breast Cancer: Today's post is brought to you by the letter 'B'
The transcription factor beta protein 1 (BP1) is a member of the homeobox gene family and the distal-less subfamily. Expression of BP1 is highly tissue-specific and developmentally restricted. Among different human tissues, BP1 is found to be highly e...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol DLX4

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