BP1 Antibody

Images

 
Western Blot: BP1 Antibody [NB100-481] - Detection of MDA-MB-231 cell lysates.
Immunohistochemistry-Frozen: BP1 Antibody [NB100-481] - Top Panel: BP1 antibody detected in breast cancer cells. Bottom Panel: Staining of normal breast tissue.
Immunoprecipitation: BP1 Antibody [NB100-481] - Antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 ug of cell extract using anti-BP1 (NB100-481) antibody diluted 1:1,000 3. Negative ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

BP1 Antibody Summary

Immunogen
A synthetic peptide made specifically to the human BP1 protein sequence (between residues 1-60) [UniProt# Q92988].
Localization
Nuclear.
Specificity
This is specific for human BP1 (DLX4 variant 1). Berg PE, Kirolikar S. DLX4 (distal-less homeobox 4). Atlas Genet Cytogenet Oncol Haematol. January 2011. URL : http://AtlasGeneticsOncology.org/Genes/DLX4ID49827ch17q21.html
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLX4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This BP1 antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western blot, where a band is seen at ~36 kDa. IP has been done with 35S-labeled BP1 protein only. It is not known if this antibody will immunoprecipitate a cell extract. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
BP1 Lysate (NBL1-09919)
Reviewed Applications
Read 1 Review rated 4
using
NB100-481 in the following applications:

Publications
Read Publications using
NB100-481 in the following applications:

  • IHC
    1 publication
  • WB
    4 publications

Reactivity Notes

Immunogen sequence has 82% homology with mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BP1 Antibody

  • Beta protein 1
  • BP1distal-less homeo box 7
  • distal-less homeobox 4
  • DLX7distal-less homeo box 9
  • DLX8DLX9
  • homeobox protein DLX-4
  • Homeobox protein DLX-7
  • Homeobox protein DLX-8

Background

BP1 (beta protein 1; also called distal-less homeobox gene 4 or DLX4) is a member of DLX family of homeobox genes and it controls many aspects of embryonic development including bone morphogenesis, skeletal patterning and cell differentiation. Originally discovered to be a repressor of beta-globin gene in erythroid cells, BP1 is normally expressed during early hematopoiesis with complete absence from most normal adult tissues and is widely re-expressed in leukemias, lung, breast, ovarian as well as prostate cancers. BP1 is an oncogene with a major role in breast cancer, and that its expression correlates with breast cancer progression and invasion. BP1 has been found to be detectable in 80% of infiltrating ductal carcinomas, but undetectable in matched normal controls, being the first DLX gene to be strongly implicated in breast cancer. Furthermore, expression of DLX4 correlates with estrogen receptor status and also cancer progression and invasion in breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF674
Species: Hu
Applications: WB, Simple Western, Neut
NBP2-24646
Species: Hu, Mu, Bv, Ca, Eq, Pm
Applications: WB, IHC-P
NBP2-14081
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00001746-M06
Species: Hu, Mu
Applications: WB, ELISA, IHC-P
NBP1-85793
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-47492
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
H00001745-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NBP1-89719
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85929
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-85445
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NB200-157
Species: Hu
Applications: WB, IHC, IHC-P, KO
NB300-560
Species: Hu, Mu, Rt, Av, Bv, Pm
Applications: WB, IHC, IHC-Fr, IHC-P, IF
NBP1-89296
Species: Hu
Applications: WB, IHC, IHC-P
NB100-481
Species: Hu
Applications: WB, IHC, IHC-Fr, IHC-P, IP

Publications for BP1 Antibody (NB100-481)(6)

Review for BP1 Antibody (NB100-481) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-481:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Patricia Berg
WB Human 08/10/2010
View

Summary

ApplicationWestern Blot
Sample Testedwhole cell lysate, Sample Amount: 10-20ug/well
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% in TNET, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:5000, Incubation Dilution Buffer: TNET, Incubation Time: overnight, Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-Rabbit, Secondary Ab Dilution Ratio: 1:15000
Secondary Manufacturer Cat#Pierce #1858415

Details

Detection NotesDetection Method: Chemiluminescence, Exposure Time: 5-30 min, Specific Bands: 37 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BP1 Antibody (NB100-481) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-481AF405
Alexa Fluor 488 Labeled NB100-481AF488
Alexa Fluor 647 Labeled NB100-481AF647
Alexa Fluor 700 Labeled NB100-481AF700
Biotin Labeled NB100-481B
DyLight 405 Labeled NB100-481V
DyLight 488 Labeled NB100-481G
DyLight 550 Labeled NB100-481R
DyLight 650 Labeled NB100-481C
FITC Labeled NB100-481F
HRP Labeled NB100-481H

Additional Array Products

Bioinformatics Tool for BP1 Antibody (NB100-481)

Discover related pathways, diseases and genes to BP1 Antibody (NB100-481). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BP1 Antibody (NB100-481)

Discover more about diseases related to BP1 Antibody (NB100-481).
 

Pathways for BP1 Antibody (NB100-481)

View related products by pathway.

PTMs for BP1 Antibody (NB100-481)

Learn more about PTMs related to BP1 Antibody (NB100-481).
 

Research Areas for BP1 Antibody (NB100-481)

Find related products by research area.

Blogs on BP1.

BRCA1 - A Critical Tumor Suppressor Gene in Women
Breast cancer 1, early onset (BRCA1) is a well-known tumor suppressor gene that was originally discovered due to its link with early-onset breast and ovarian cancer in women. The BRCA1 protein contains the following domains: RING finger, RAD51-interac...  Read full blog post.

BP1 Antibodies, Beta Globin and Breast Cancer: Today's post is brought to you by the letter 'B'
The transcription factor beta protein 1 (BP1) is a member of the homeobox gene family and the distal-less subfamily. Expression of BP1 is highly tissue-specific and developmentally restricted. Among different human tissues, BP1 is found to be highly e...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Patricia Berg
08/10/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol DLX4
Entrez
Uniprot