BMP-4 Antibody (3C11C7) - BSA Free

Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mouse mAb against A549 (1), HepG2 (2), and C6 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis of Hela cells using BMP4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow Cytometry: BMP-4 Antibody (3C11C7) [NBP2-52424] - Flow cytometric analysis of Hela cells using BMP4 mouse mAb (green) and negative control (red).

Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against human BMP4 recombinant protein. (Expected MW is 41 kDa)

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11C7) [NBP2-52424] - Immunofluorescence analysis of Hela cells using BMP4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

ELISA: BMP-4 Antibody (3C11C7) [NBP2-52424] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 3C11C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BMP-4 Antibody (3C11C7) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human BMP-4 (AA: 277-408) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BMP4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready. IHC reported in scientific literature (PMID: 29306659).
• Theoretical MW: 46.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for BMP-4 Antibody (3C11C7) - BSA Free

• BMP2B
• BMP-2B
• BMP2B1
• BMP2BMCOPS6
• BMP4
• BMP-4
• Bone morphogenetic protein 2B
• bone morphogenetic protein 4
• DVR4
• MCOPS6
• OFC11
• ZYME

Background

Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Through molecular cloning, 7 BMP cDNAs, designated BMP1 through BMP7, have been recovered. BMP2 through BMP7, are members of the transforming growth factor-beta superfamily of regulatory molecules. Transcript variants utilizing alternative promoters and alternative non-coding first exons have been described in the literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMP-4 Antibody (NBP2-52424)(2)

Publications using NBP2-52424

• Scimeca M, Bonfiglio R, Menichini E, et al. Microcalcifications Drive Breast Cancer Occurrence and Development by Macrophage-Mediated Epithelial to Mesenchymal Transition Int J Mol Sci 2019-11-11 [PMID: 31718020] (IHC-P, Human)
• Scimeca M, Antonacci C, Toschi N et al. Breast Osteoblast-like cells a reliable early marker for bone metastases from breast cancer. Clinical Breast Cancer 2017-12-05 [PMID: 29306659] (Human)

Bioinformatics

• Gene Symbol: BMP4