Recombinant Human BMP-2 Protein

Product Discontinued

Recombinant Human BMP-2 Protein Summary

• Description: Recombinant biologically active BMP2 protein.

  Source:E. coli

  Amino Acid Sequence:MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

• Preparation Method: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard.
• Details of Functionality: BMP-2 is fully biologically active when compared to standard.The ED50 as determined by its ability to induce alkaline phosphatase production by ATDC-5 cells is 0.5-1.0 ug/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: BMP2
• Purity: >95%, by HPLC.
• Endotoxin Note: Less than 0.1 ng/ug (IEU/ug) of Bone Morphogenetic protein-2 .

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a concentrated sterile solution containing 10mM sodium citrate pH 3.5.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by HPLC.
• Reconstitution Instructions: Reconstitute with sterilized 20 mM Acetic acid to a final concentration of at least 0.1 mg/ml.

Notes

After reconstitution, store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for Recombinant Human BMP-2 Protein

• BDA2
• BMP2
• BMP-2
• BMP-2A
• BMP2ABone morphogenetic protein 2A
• bone morphogenetic protein 2
• SSFSC

Background

BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia (myositis) ossificans progressiva.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for BMP-2 Recombinant Protein (NBP2-26568). (Showing 1 - 1 of 1 FAQ).

1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
   • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.

Bioinformatics

• Gene Symbol: BMP2