BMP-2 Antibody (208301) [Alexa Fluor® 700]

• Reactivity: Ze
• Applications: WB, B/N
• Clone: 208301
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

BMP-2 Antibody (208301) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant zebrafish BMP‑2
  Gln272-Arg386
  Accession # AAI63048
• Specificity: Detects zebrafish BMP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human BMP-2, -3, -3B,
  -4, -5, -6, -7, -8, or recombinant zebrafish BMP-4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-2 Antibody (208301) [Alexa Fluor® 700]

• BDA2
• BMP2
• BMP-2
• BMP-2A
• BMP2ABone morphogenetic protein 2A
• bone morphogenetic protein 2
• SSFSC

Background

BMP-2 is one of at least 20 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF‑ beta ) superfamily (1, 2). BMPs were originally identified as protein regulators of cartilage and bone formation. However, they are also involved in embryogenesis and morphogenesis of various tissues and organs. BMPs regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human, mouse, and rat BMP-2 share 100% amino acid (aa) sequence identity, while mature human BMP-2 and zebrafish BMP-2 share 85% aa identity. Zebrafish have another homolog of BMP-2 (also called BMP-2a) called BMP-2b, which is 88% identical to BMP-2 in the mature region and corresponds to the swirl mutant. The combined expression pattern of zfBMP-2a/2b/4 coincides with areas where BMP-2/4 expression would be found in other vertebrates (3). Biologically active BMP-2 is a disulfide-linked homodimer of the carboxy-terminal 115 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-2 have been shown to be mediated by the formation of hetero‑ogliomeric complexes of type I and type II serine/threonine kinase receptors (4). In contrast to the TGF-beta type I receptor, which does not bind the ligand in the absence of the TGF-beta receptor type II, both type I BMP receptor’s can bind BMP-2 with high affinity in the absence of BMP receptor type II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for BMP-2 Antibody (FAB111N). (Showing 1 - 1 of 1 FAQ).

1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
   • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.

Bioinformatics

• Uniprot:
  • AAI63048()