BMP-2 Antibody (1A11)

Images

 
Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody (1A11) [H00000650-M02] - IF analysis of BMP2 in zebra finch brain tissue. Image courtesy of product review submitted by Brad Walters.
ELISA: BMP-2 Antibody (1A11) [H00000650-M02] - Detection limit for recombinant GST tagged BMP2 is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, AvSpecies Glossary
Applications ELISA, IHC
Clone
1A11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

BMP-2 Antibody (1A11) Summary

Immunogen
BMP2 (NP_001191 283 a.a. - 396 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR
Specificity
BMP2 - bone morphogenetic protein 2
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BMP2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
Application Notes
Antibody reactivity against recombinant protein on ELISA. IHC usage reported in a customer review.
Reviewed Applications
Read 1 Review rated 4
using
H00000650-M02 in the following applications:

Publications
Read Publication using H00000650-M02.

Reactivity Notes

Human and Zebra Finch reactivities reported from customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BMP-2 Antibody (1A11)

  • BMP2
  • BMP-2
  • BMP-2A
  • BMP2ABone morphogenetic protein 2A
  • bone morphogenetic protein 2

Background

The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00000650-M02
Species: Hu, Av
Applications: ELISA, IHC

Publications for BMP-2 Antibody (H00000650-M02)(1)

Review for BMP-2 Antibody (H00000650-M02) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using H00000650-M02:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry BMP-2 H00000650-M02
Enlarge
reviewed by:
Anonymous
ICC Human 05/06/2010
View

Summary

ApplicationImmunocytochemistry
Sample TestedHeLa whole cell, mito extract, Sample Amount: 20ug
SpeciesHuman
Lot7184-1A11-00NBY6
Comments"Recognizes at least 3 bands = different cleavage products before and after stabilization.
Cross-reacting band in my HeLa cells just above the top PINK1 band."

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: 5%milk in TBST, Incubation Time: 1 hour to overnight, Incubation Temp: RT

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit HRP, Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method:ECL, Exposure Time:brief, Neg.Cntl:PINK1 siRNA, Bands:3 @ correct sizes(specific), one @ 65-70kDa(non-specific)

Comments

Comments"Recognizes at least 3 bands = different cleavage products before and after stabilization.
Cross-reacting band in my HeLa cells just above the top PINK1 band."

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for BMP-2 Antibody (H00000650-M02). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
    • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for BMP-2 Antibody (H00000650-M02)

Discover related pathways, diseases and genes to BMP-2 Antibody (H00000650-M02). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for BMP-2 Antibody (H00000650-M02)

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Blogs on BMP-2

There are no specific blogs for BMP-2, but you can read our latest blog posts.

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Contact Information

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Recent Reviews

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Anonymous
05/06/2010
Application: ICC
Species: Human

Bioinformatics

Gene Symbol BMP2
Entrez
OMIM
Uniprot