Bmf Antibody (17A9) - BSA Free

Flow Cytometry: Bmf Antibody (17A9) - BSA Free [NBP2-80091] - Data of overexpressed EE-tagged Bmf in 293T cells, fixed with 1% PFA, permeabilized with saponin and stained intracellularly with Bmf (mouse/rat), mAb (17A9) (Prod. No. NBP2-80091)

Bmf Antibody (17A9) - BSA Free [NBP2-80091] - Using Bmf (mouse/rat), mAb (17A9) (Prod. No. NBP2-80091) detecting endogenous Bmf in mouse spleen, lymph node and thymus as two bands of ~25 and ~30 kDa, but not in tissues from two knock-out Bmf strains del 372 and del 376.

Immunoprecipitation: Bmf Antibody (17A9) - BSA Free [NBP2-80091] - Of endogenous Bmf from mouse spleen cells using MAb to Bmf (17A9) (Prod. No. NBP2-80091).

• Reactivity: Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, KO
• Clone: 17A9
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Bmf Antibody (17A9) - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rat Bmf Antibody (17A9) - BSA Free (NBP2-80091) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant full length mouse Bmf and synthetic peptides corresponding to aa 13-27 and aa 112-126 of mouse Bmf.
• Specificity: Recognizes an epitope within the peptide sequence aa 13-27 of mouse and rat Bmf. Detects bands of ~25kDa and ~30kDa by Western blot, corresponding to endogenous Bmf and BmfL. Does not cross-react with human Bmf.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: BMF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Bmf Antibody (17A9) - BSA Free

• Bcl2 modifying factor
• bcl-2-modifying factor
• FLJ00065

Background

Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl-2 family members, among others, play an important role.The Bcl-2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Members of this family include both pro- and anti-apoptotic proteins that hare homology sequences called Bcl-2 Homology domains (BH1-4) which mediate dimmer formation. The BH3 proteins, such as BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence homology within the amphipathic alpha-helical BH3 region, which is required for their apoptotic function. They may trigger release of death-inducing molecules such as Cytochrome C, Smac, and endonuclease G. Anti-apoptotic family members, including Bcl-2 and Bcl-XL, play inhibitory roles. Bcl-2 family proteins may form homodimers or heterodimers between pro- and anti-apoptotic members, the ratios of which determine the cell fate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BMF